Rapid monitoring assay of congenital adrenal hyperplasia with microbore high-performance liquid chromatography/electrospray ionization tandem mass spectrometry from dried blood spots

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Title:	Rapid monitoring assay of congenital adrenal hyperplasia with microbore high-performance liquid chromatography/electrospray ionization tandem mass spectrometry from dried blood spots
Authors:	Lai, CC;Tsai, CH;Tsai, FJ;Lee, CC;Lin, WD
Contributors:	附設醫院基因醫學部;China Med Coll Hosp, Dept Med Genet & Med Res, Taichung 404, Taiwan
Date:	2001
Issue Date:	2010-09-24 14:45:12 (UTC+8)
Publisher:	JOHN WILEY & SONS LTD
Abstract:	Some patients with chronic hepatitis C respond to interferon (IFN)-alpha treatment, and the efficiency can be improved by combining it with ribavirin. The mechanism of this improvement is unknown. To investigate the effects of these two regimens on the immune responses in 51 patients with chronic hepatitis C, we examined the hepatitis C core antigen-specific proliferative response and cytokine production profiles, natural killer (NK) cell cytotoxicity and cytotoxic T cell function during treatment. The results are as follows: (1) both viral clearance and biochemical normalization occurred more frequently in patients receiving combination therapy; (2) the function of NK cells increased after treatment in the responders of both groups (p < 0.05); (3) the level of IFN-gamma produced by hepatitis C core antigen-stimulated peripheral blood mononuclear cells was higher in patients receiving combination therapy, especially in responders; (4) the core antigen-specific proliferative response decreased after treatment, and (5) in addition, the core-specific cytotoxic T cell activities of five responder patients also increased significantly after therapy. In conclusion, enhancement of immune responses, especially those related to type-1 T helper cell activity, may contribute to better efficacy in combining ribavirin with IFN-alpha for treatment of chronic hepatitis C. Copyright (C) 2001 National Science Council, ROC and S. Karger AG, Basel.
Relation:	RAPID COMMUNICATIONS IN MASS SPECTROMETRY 15(22):2145-2151
Appears in Collections:	[China Medical University Hospital] Jurnal articles

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