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| 題名: | Lack of evidence for the association of tumor necrosis factor-alpha gene promoter polymorphism with calcium oxalate stone and bladder cancer patients |
| 作者: | Tsai, FJ;Lu, HF;Yeh, LS;Hsu, CD;Chen, WC |
| 貢獻者: | 附設醫院基因醫學部;China Med Coll, China Med Coll Hosp, Dept Med Genet, Taichung, Taiwan;China Med Coll, China Med Coll Hosp, Dept Pediat, Taichung, Taiwan;China Med Coll, China Med Coll Hosp, Dept Urol, Taichung, Taiwan;China Med Coll, China Med Coll Hosp, Dept Obstet & Gynecol, Taichung, Taiwan |
| 日期: | 2001 |
| 上傳時間: | 2010-09-24 14:45:08 (UTC+8) |
| 出版者: | SPRINGER-VERLAG |
| 摘要: | Purpose: To evaluate the activities of acetyl coenzyme A (AcCoA): arylamine N-acetyltransferase (NAT) of intact cumulus granulosa cells and the role of leukemia inhibitory, factor (LIF) upon their NAT activities. Methods. Thirty women accepted controlled ovarian hyper-stimulation (COH) and oocyte retrievals. Human cumulus granulosa cells were obtained during oocyte retrievals. Using 2-aminofluorene (2-AF) and p-aminobenzoic acid (PABA) its substrates, NAT activity of till samples was determined by high pressure liquid chromatography. After the incubation with different time and concentrations of 2-AF FABA, and LIF 2-acetyl-aminofluorene (2-AAF) and N-acetyl-PABA (N-Ac-PABA) were measured. Results: After incubation with 2.812, 5.625, 11.25, and 22.5 muM of 2-AF/PABA, their product concentrations (2-AAFIN-Ac-PABA) were 0.42/0.32, 0.76/0.58, 1.29/1.04, and 1.94/1.26 nmol/10(6) cells, respectively,. After 6, 12, 18, and 24 It incubation with 11.25 muM of 2-AF/PABA, their product concentrations were 0.19/0.12, 0.56/0.4, 0.98/0.79. and 1.3/1.0 nmol/10(6) cells, respectively. After incubation with 0, 5, and 50muM of LIF the 2-AAFIN-Ac-PABA concentrations were 0.98/0.80, 0.70/0.52, and 0.49/0.30 nmol/10(6) cells, respectively. Conclusion. Intact human cumulus granulosa Cells could acetylate arylamine carcinogen (2-AF) and noncarcinogens drug (PABA). LIF decreased the NAT activities. It provides a model for monitoring the effects of COH and LIF upon the oocytes. |
| 關聯: | UROLOGICAL RESEARCH 29(6):412-416 |
| 顯示於類別: | [台中附設醫院] 期刊論文
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