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請使用永久網址來引用或連結此文件:
http://ir.cmu.edu.tw/ir/handle/310903500/29875
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題名: | Effect of anti-inflammatory medication on monocyte response to titanium particles |
作者: | Sun, JS;Lin, FH;Tsuang, YH;Chen, LT;Hong, RC;Chang, WHS;Liu, HC |
貢獻者: | 附設醫院骨科部;Chung Yuan Christian Univ, Inst Biomed Engn, Tao Yuan, Taiwan;Natl Taiwan Univ Hosp, Dept Orthoped Surg, Taipei 10002, Taiwan;Natl Taiwan Univ, Coll Med, Ctr Biomed Engn, Taipei, Taiwan;China Med Coll Hosp, Dept Orthoped Surg, Taichung, Taiwan |
日期: | 2000 |
上傳時間: | 2010-09-24 14:44:32 (UTC+8) |
出版者: | JOHN WILEY & SONS INC |
摘要: | Purpose: To assess the influence of different concentrations of recombinant human leukemia inhibitory factor (LIF) on the in vitro development of mouse embryos. Methods: The 2- to 4-cell embryos of CB6F1 mice were cultured in the human tubal fluid (HTF) media containing different concentrations of LIF. Mouse embryos were divided into seven groups: (1) HTF; (2) 1500 IU/ml LIF; (3) 1000 IU/ml LIF; (4) 750 IU/ml LIF; (5) 500 IU/ml LIF; (6) 250 IU/ml LIF; (7) 125 IU/ml LIF. The embryonic numbers of different stages including 5-8 cell, 9-16 cell, morula, blastocyst, and hatching blastocyst were recorded. Results: The percentage of early embryo stage (2-cell embryos to 6- to 16-cell stages) in all groups were nonsignificantly different. There were higher formation rates of preimplantation embryos (morula to hatching blastocyst) in groups 2, 3, 4, and 5 than in groups 1, 6 and 7. Conclusions: LIF has positive effects on preimplantation embryo development and has nonsignificant influence on the early embryo development. The lowest concentration of LIF which could provide the optimal embryo development is 500 IU/ml. |
關聯: | JOURNAL OF BIOMEDICAL MATERIALS RESEARCH 52(3):509-516 |
顯示於類別: | [台中附設醫院] 期刊論文
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