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http://ir.cmu.edu.tw/ir/handle/310903500/29832
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| 題名: | Modification of superoxide dismutase (SOD) mRNA and activity by a transient hypoxic stress in cultured glial cells |
| 作者: | Niu, CS;Chang, CK;Lin, LS;Jou, SB;Kuo, DH;Liao, SS;Cheng, JT |
| 貢獻者: | 附設醫院神經部;Natl Cheng Kung Univ, Coll Med, Dept Pharmacol, Tainan 70101, Taiwan;Tajen Jr Coll Pharm, Dept Pharm, Pingtung 90703, Taiwan;China Med Coll Hosp, Dept Neurol, Taichung 40408, Taiwan;Mackay Mem Hosp, Dept Surg, Div Neurosurg, Taipei 10401, Taiwan;Tzu Chi Jr Coll Nursing, Dept Pharmacol, Hualien 97001, Taiwan |
| 日期: | 1998 |
| 上傳時間: | 2010-09-24 14:43:28 (UTC+8) |
| 出版者: | ELSEVIER SCI IRELAND LTD |
| 摘要: | Arylamine N-acetyltransferase (NAT) activity in Pseudomonas aeruginosa was inhibited by sulindac, a drug proposed for cancer prevention. By using high performance liquid chromatography, the NAT activity for acetylation of 2-aminofluorene (2-AF) was examined. The cytosolic NAT activity in P. aeruginosa ATCC 27853 was 5.32 +/- 0.36 nmol/min/mg of protein. Sulindac displayed a dose-dependent inhibition to cytosolic NAT activity in P. aeruginosa with an IC50 value of about 0.46 mM. The NAI activity measured from intact P. aeruginosa cells was 1.39 +/- 0.12 nmol/min/10(10) CFU and this activity was inhibited by sulindac in a similar dose-dependent fashion with an IC50 value of similar to 0.42 mM. Time-course experiments showed that NAT activity measured from intact Fl aeruginosa cells was inhibited by sulindac for at least 3 h. Using standard steady-state kinetic analysis, it was demonstrated that sulindac was a possible noncompetitive inhibitor to NAT activity in Fl aeruginosa. The results suggest that sulindac suppressed the NAT activity in Fl aeruginosa. |
| 關聯: | NEUROSCIENCE LETTERS 251(3):145-148 |
| 顯示於類別: | [台中附設醫院] 期刊論文
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