Cage containing a biphasic calcium phosphate ceramic (Triosite) for the treatment of cervical spondylosis

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Title:	Cage containing a biphasic calcium phosphate ceramic (Triosite) for the treatment of cervical spondylosis
Authors:	Cho, DY;Lee, WY;Sheu, PC;Chen, CC
Contributors:	附設醫院神經外科;China Med Univ Hosp, Dept Neurosurg, Taichung 404, Taiwan
Date:	2005
Issue Date:	2010-09-24 14:43:07 (UTC+8)
Publisher:	ELSEVIER SCIENCE INC
Abstract:	The bi-functional enzyme, adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase (CobU), is involved in the biosynthesis of cobalamin in Salmonella typhimurium. and, therefore. can be used for the in vitro synthesis of analogs of B-12. Previously, five different steps were required to purify the recombinant enzyme from Escherichia coli. Here. we describe the cloning, sequencing, and expression of the cobU gene from S. typhimurium ATCC 19585 and, without introducing a purification tag sequence to the N- or C-terminus of the recombinant enzyme, a new single-step purification method based on hydrophobic interaction chromatography. (c) 2005 Elsevier Inc. All rights reserved.
Relation:	SURGICAL NEUROLOGY 63(6):497-504
Appears in Collections:	[China Medical University Hospital] Jurnal articles

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