Predicting multidrug resistance-related protein and P-glycoprotein expression with technetium-99m tetrofosmin mammoscintigraphy

中國醫藥大學機構典藏 China Medical University Repository, Taiwan > 台中附設醫院 > 期刊論文 > Item 310903500/29720

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题名:	Predicting multidrug resistance-related protein and P-glycoprotein expression with technetium-99m tetrofosmin mammoscintigraphy
作者:	Liu, TJ;Shiau, YC;Tsai, SC;Wang, JJ;Ho, ST;Kao, A
贡献者:	附設醫院核子醫學部;China Med Coll Hosp, Dept Nucl Med, Taichung 404, Taiwan;China Med Coll Hosp, Dept Med Res, Taichung 404, Taiwan;Natl Def Med Ctr, Sch Med, Taipei, Taiwan;Chi Mei Med Ctr, Dept Med Res, Tainan, Taiwan;Show Chwan Mem Hosp, Dept Nucl Med, Chunghua, Taiwan;Natl Taiwan Univ, Coll Elect Engn, Inst Biomed Engn, Far Eastern Mem Hosp Dept Nucl Med, Taipei 10764, Taiwan;Taichung Vet Gen Hosp, Dept Surg, Taichung, Taiwan
日期:	2003
上传时间:	2010-09-24 14:41:12 (UTC+8)
出版者:	CHURCHILL LIVINGSTONE
摘要:	Arylamine N-acetyltransferase (NAT) plays an important role in the first step of arylamines metabolism. Luteolin has shown antibactrial, antioxidant and antineoplastic activity. To glean insights into the mechanism of action of luteolin, we assessed the effects of luteolin on NAT activity and gene expression and DNA-2-aminofluorene (AF) adduct formation in human bladder cancer T24 cells. By using high performance liquid chromatography, the amounts of N-acetylation of AF and DNA adducts were determined and quantitated. Gene expression was performed by using polymerase chain reaction and gel electrophoresis. The results indicated 1) luteolin displayed a dose-dependent inhibition of NAT activity and gene expression (NAT1 mRNA) in T24 cells; 2) time-course experiments showed that N-acelylation of AF measured from intact T24 cells was inhibited by luteolin for up to 48 hours; 3) using standard steady-state kinetic analysis, it was demonstrated that luteolin was a possible uncompetitive inhibitor of NAT activity in T24 cells; and 4) the DNA-AF adduct formation in T24 cells was inhibited by luteolin. This report is the first finding which has shown luteolin to affect human T24 cell NAT activity and gene expression (NAT1 mRNA) and DNA-AF adduct formation.
關聯:	BREAST 12(1):58-62
显示于类别:	[台中附設醫院] 期刊論文

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