Using thallium-201 spect to detect recurrent or residual nasopharyngeal carcinoma after radiotherapy in patients with indeterminate CT findings

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Title:	Using thallium-201 spect to detect recurrent or residual nasopharyngeal carcinoma after radiotherapy in patients with indeterminate CT findings
Authors:	Shiau, YC;Liu, FY;Huang, WS;Yen, RF;Kao, CH
Contributors:	附設醫院核子醫學部;China Med Coll Hosp, Dept Nucl Med, Taichung 404, Taiwan;Far Eastern Mem, Dept Nucl Med, Taichung, Taiwan;Triserv Gen Hosp, Dept Nucl Med, Taipei, Taiwan;Triserv Gen Hosp, Natl Def Med Ctr, Taipei, Taiwan;Natl Taiwan Univ Hosp, Dept Nucl Med, Taipei, Taiwan
Date:	2003
Issue Date:	2010-09-24 14:40:27 (UTC+8)
Publisher:	JOHN WILEY & SONS INC
Abstract:	We examined ultraviolet (UV) irradiation and cisplatin treatment damage, formation and repair efficiency in the p53 tumor suppressor gene of various cultured cell lines and lymphocytes using a nonradioactive multiplex long quantitative polymerase chain reaction (QPCR) assay, which amplified a 7-kb fragment of the target gene and a 500-bp fragment of the template control to successfully increase the sensitivity and reliability of the assay. The multiplex long QPCR detected a lesion frequency of 0.63 lesions/10 kb/10J/m(2) 2 in the p53 gene of fibroblast cells. In addition, the multiplex long QPCR assay detected pronounced differences in the repair of UV damage in the p53 gene among repair-proficient CRL-1475 cells and repair-deficient XP-A and XP-C cells. The multiplex long QPCR assay was also evaluated as a sensitive assay for the detection of DNA damage induced by cisplatin. The data indicated that the lesion frequency in the p53 gene was 1.27-1.75 times higher in the H23 cisplatin-sensitive cell than in the H1435 cisplatin-resistant cell at the IC70 dose. After 8-h and 24-h repair periods, only 13 and 75% of cisplatin-induced damage had been removed in the H23 cells, whereas these values were 92 and 100% in the H1435 cells. In addition, our data indicate that multiplex long QPCR is a sensitive method for validly estimating repair in freshly isolated lymphocytes. The results suggest that the current protocol of the multiplex long QPCR method can be used to assess the damage formation and repair efficiency of various agents at biologically relevant doses and to allow a more precise determination of gene-specific repair in disease susceptibility and drug resistance in epidemiological studies. (C) 2003 Elsevier Science (USA). All rights reserved.
Relation:	HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK 25(8):645-648
Appears in Collections:	[China Medical University Hospital] Jurnal articles

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