Evaluation of nasal mucociliary clearance function in allergic rhinitis patients with technetium 99m-labeled macroaggregated albumin rhinoscintigraphy

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Title:	Evaluation of nasal mucociliary clearance function in allergic rhinitis patients with technetium 99m-labeled macroaggregated albumin rhinoscintigraphy
Authors:	Sun, SS;Hsieh, JF;Tsai, SC;Ho, YJ;Kao, CH
Contributors:	附設醫院核子醫學部;China Med Coll Hosp, Dept Nucl Med, Taichung 404, Taiwan;Jen Ai Hosp, Dept Radiol, Taichung, Taiwan;Chi Mei Fdn Hosp, Dept Nucl Med, Tainan, Taiwan;Show Chwan Mem Hosp, Dept Nucl Med, Chunghua, Taiwan
Date:	2002
Issue Date:	2010-09-24 14:39:36 (UTC+8)
Publisher:	ANNALS PUBL CO
Abstract:	The reverse transcriptase-polymerase chain reaction (RTPCR) technique is a tool capable of detecting minute quantities of circulating tumor cell-derived transcripts. Nasopharyngeal carcinoma (NPC) is a rapidly growing tumor of epithelial origin and high metastatic potential. The aim of our study is to investigate the clinical value of circulating cytokeratin-19 (CK-19) mRNA detection in NPC patients. Between June 1997 and March 1999, 57 previously untreated, advanced NPC patients without distant metastasis were uniformly treated by concurrent chemoradiotherapy. Peripheral blood samples were collected prospectively before treatment and subjected to a nested RT-PCR assay. Measures were taken to prevent contamination and pseudogene interference. PCR products of positive results were verified by restriction enzyme Hae II and direct sequencing. Under our nested RT-PCR experimental conditions, 33.3% (19/57) clinically nonmetastatic NPC patients had CK-19 mRNA in their blood. The positive detection rates of CK-19 mRNA in the peripheral blood for different stages were 20.0% for stage II, 31.6% stage III and 43.5% stage IV (p=0.1335). After a median follow-up time of 35 months, 2 patients had recurrences of their primary tumors and 14 developed distant metastases without locoregional recurrence. Nine of 19 (47.4%) CK-19 mRNA-positive patients and 5 of 38 (13.2%) CK-19 mRNA-negative patients developed distant metastasis (p=0.00826). The 3-year metastasis-free survival rates were 49.9% for patients with detectable CK-19 and 85.9% for those with undetectable CK-19 (p=0.0089, log-rank test). Our data suggest that the presence of CK-19 mRNA in the peripheral blood may be a potential marker of micrometastasis for NPC. (C) 2002 Wiley-Liss, Inc.
Relation:	ANNALS OF OTOLOGY RHINOLOGY AND LARYNGOLOGY 111(1):77-79
Appears in Collections:	[China Medical University Hospital] Jurnal articles

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