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http://ir.cmu.edu.tw/ir/handle/310903500/29644
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| 題名: | Discordance between dipyridamole stress Tc-99m sestamibi SPECT and coronary angiography in patients with Kawasaki disease |
| 作者: | Fu, YC;Kao, CH;Hwang, B;Jan, SL;Chi, CS |
| 貢獻者: | 附設醫院核子醫學部;Kao, CH, China Med Coll Hosp, Dept Nucl Med, 2 Yuh Der Rd, Taichung 404, Taiwan |
| 日期: | 2002 |
| 上傳時間: | 2010-09-24 14:39:28 (UTC+8) |
| 出版者: | MOSBY, INC |
| 摘要: | The potential residue at the autoprocessing site for improving processing efficiency was evaluated from hydrolysis of 19 cleavage-site-mimicking octapeptides, VTTXQTVP (-4 to +4), by the mature subtilisin YaB and YaB-G124A mutants. Both enzymes cleaved the octapeptides mainly at two sites, X-Q (A-site) and Q-T (B-site), at varied preferences. Based on the results above, Met(-1) of YaB-G124A was mutated and, as expected, extracellular enzyme production increased with Gin or Ala replacement, but decreased with Ile or Asp substitution. Together with previous structural studies, our results suggest that autoprocessing is dependent on not only the primary structure, but also the peptide flexibility around the processing site. Cleavage at the B-site resulted in a novel YaB mutant lacking the N-terminus Gln 1, which led the mutant enzyme to less enzymatic activity by 80% and less thermal stability by 20degreesC, perhaps due to its ligation to the high-affinity calcium ion. (C) 2002 Elsevier Science (USA). |
| 關聯: | JOURNAL OF NUCLEAR CARDIOLOGY 9(1):41-46 |
| 顯示於類別: | [台中附設醫院] 期刊論文
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