Clp upregulates transcription of engA gene encoding a virulence factor in Xanthomonas campestris by direct binding to the upstream tandem Clp sites

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Title:	Clp upregulates transcription of engA gene encoding a virulence factor in Xanthomonas campestris by direct binding to the upstream tandem Clp sites
Authors:	Hsiao, YM;Liao, HY;Lee, MC;Yang, TC;Tseng, YH
Contributors:	健康照護學院醫技系;Chungtai Inst Hlth Sci & Technol, Inst Med Biotechnol, Taichung 406, Taiwan;Taiwan Agr Chem & Tox Substances Res Inst, Dept Pesticide Chem, Taichung 413, Taiwan;China Med Univ, Dept Med Technol, Taichung 404, Taiwan;Natl Chung Hsing Univ, Inst Mol Biol, Taichung 402, Taiwan
Date:	2005
Issue Date:	2010-09-24 14:39:07 (UTC+8)
Publisher:	ELSEVIER SCIENCE BV
Abstract:	Autologous chondrocyte transplantation (ACT) is a promising method to treat chondral and osteochondral defects. This study introduced a modified method for cell culture in ACT. Porcine chondrocytes were cultured for 3 weeks under low hydrostatic pressure at 250 Pa. The results showed that the dry weight of the cartilage-like membrane in the loading group was 3.0 times more than the control group (no loading) (p < 0.01), and cell numbers were significantly increased by 3.1 times (p < 0.01) after a 3-week culture. Compared with the fresh tissue sample, the mRNA expression of collagen II was not statistically different and the mRNA of aggrecan was only slightly decreased by 19%. These data suggest that the hydrostatic pressure at this level significantly increased the cell numbers and biosynthesis of cultured chondrocytes.
Relation:	FEBS LETTERS 579(17):3525-3533
Appears in Collections:	[Department of Medical Laboratory Science and Biotechnology ] Journal articles

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