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| 題名: | Characterization of trans- and cis-cleavage activity of the SARS coronavirus 3CL(pro) protease: basis for the in vitro screening of anti-SARS drugs |
| 作者: | Lin, CW;Tsai, CH;Tsai, FJ;Chen, PJ;Lai, CC;Wan, L;Chiu, HH;Lin, KH |
| 貢獻者: | 健康照護學院醫技系;China Med Univ, Dept Med Lab Sci & Biotechnol, Taichung 404, Taiwan;China Med Univ Hosp, Dept Med Genet & Med Res, Taichung 404, Taiwan;China Med Univ Hosp, Dept Lab Med, Clin Virol Lab, Taichung 404, Taiwan;Natl Taiwan Univ Hosp, Coll Med, Dept Internal Med, Taipei 100, Taiwan |
| 日期: | 2004 |
| 上傳時間: | 2010-09-24 14:38:47 (UTC+8) |
| 出版者: | ELSEVIER SCIENCE BV |
| 摘要: | Klebsiella oxytoca has been shown to be able to grow on 1-8 mM of tetracyanonickelate (II) [K2Ni(CN)(4)] (TCN), the sole nitrogen source. The biotransformation of 1 mM of TCN to ammonia and methane by resting cells of K. oxytoca was confirmed in this study. The utilization of 1 mM of TCN was strikingly enhanced when 0.8% of glucose was added into the phosphate buffer containing the same amount of resting cells. The growth cells under aerobic conditions had the higher capacity of degrading 1 mM of TCN than cells grown on oxygen-limiting conditions. The consumption of 1 mM of TCN by resting cells of K. oxytoca was induced when the pretreatment of these cells with 1 mM of TCN was conducted. However, the production of methane and acetylene by resting cells pretreated with ammonia (greater than or equal to1 mM) was inhibited. Thus, ammonia concentrations would affect the degradation of TCN by resting cells of K. oxytoca. This might result from the suppression of nitrogenase activity of K. oxytoca by ammonia. In addition, TCN degradation did not occur in presence of nitrite. This suggests that nitrite might have the toxic effect on bacterial growth. Results from this study also show that TCN biodegradation by cell-free extracts was not observed. This might be due to the oxygen exposure, which caused the inactivation of nitrogenase (oxygen-labile enzyme). (C) 2004 Elsevier Inc. All rights reserved. |
| 關聯: | FEBS LETTERS 574(1月3日):131-137 |
| 顯示於類別: | [醫學檢驗生物技術學系暨碩士班 ] 期刊論文
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