Predicting chemotherapy response to paclitaxel-based therapy in advanced non-small-cell lung cancer (Stage IIIb or IV) with a higher T stage (> T2) - Technetium-99m methoxyisobutylisonitrile chest single photon emission computed tomography and p-glycoprotein expression

中國醫藥大學機構典藏 China Medical University Repository, Taiwan > 台中附設醫院 > 期刊論文 > Item 310903500/29573

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题名:	Predicting chemotherapy response to paclitaxel-based therapy in advanced non-small-cell lung cancer (Stage IIIb or IV) with a higher T stage (> T2) - Technetium-99m methoxyisobutylisonitrile chest single photon emission computed tomography and p-glycoprotein expression
作者:	Hsu, WH;Yen, RF;Kao, CH;Shiun, SC;Hsu, NY;Lin, CC;Lee, CC
贡献者:	附設醫院核子醫學部;China Med Coll Hosp, Dept Nucl Med, Div Pulm & Crit Care Med, Taichung 404, Taiwan;China Med Coll Hosp, Dept Med, Div Pulm & Crit Care Med, Taichung 404, Taiwan;China Med Coll Hosp, Dept Chest Surg, Div Pulm & Crit Care Med, Taichung 404, Taiwan;China Med Coll Hosp, Dept Family Med, Div Pulm & Crit Care Med, Taichung 404, Taiwan;China Med Coll Hosp, Dept Med Res, Div Pulm & Crit Care Med, Taichung 404, Taiwan;Natl Taiwan Univ Hosp, Dept Nucl Med, Taipei, Taiwan
日期:	2002
上传时间:	2010-09-24 14:38:08 (UTC+8)
出版者:	KARGER
摘要:	Studies have demonstrated that berberine exhibits the antineoplastic action in rat model. Rat glial tumor cells also have been shown to have N-acetyltransferase activity. In this study, we reported the effects of berberine on arylamine N-acetyltransferase (NAT) activity, gene expression, and DNA adduct formation in human brain tumor cell lines (G95/VGH and GBM 8401). The activity of NAT (N-acetylation of substrate) was measured and determined by high-performance liquid chromatography (HPLC) assaying for the amounts of acetylated 2-aminofluorene (AF) and nonacetylated AF. Human brain tumor cells (G9T/VGH and GBM 8401) were used for examining NAT activity and gene expression and AF-DNA adduct formation. NAT gene expression was determined by polymerase chain reaction (PCR) for the levels of mRNA NAT in both examined cells lines. The amounts of AF-DNA adducts were also determined and quantities by HPLC. The results demonstrated that NAT activity, levels of mRNA NAT1 and AF-DNA adduct formation in both examined cell were inhibited and decreased by berberine in a dose-dependent manner. The apparent values of Km and Vmax from NAT of both examined cells were also determined with or without berberine cotreatment. The data also indicated that berberine decreased the apparent values of Km and Vmax. These effects also indicate that berberine is a uncompetitive inhibitor.
關聯:	ONCOLOGY 63(2):173-179
显示于类别:	[台中附設醫院] 期刊論文

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