Objective evidence of abnormal regional cerebral blood flow in patients with systemic lupus erythematosus on Tc-99m ECD brain SPECT

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Title:	Objective evidence of abnormal regional cerebral blood flow in patients with systemic lupus erythematosus on Tc-99m ECD brain SPECT
Authors:	Huang, WS;Chiu, PY;Tsai, CH;Kao, A;Lee, CC
Contributors:	附設醫院核子醫學部;China Med Coll Hosp, Dept Nucl Med, Taichung, Taiwan;China Med Coll Hosp, Dept Med Res, Taichung, Taiwan;China Med Coll Hosp, Dept Neurol, Taichung, Taiwan
Date:	2002
Issue Date:	2010-09-24 14:38:02 (UTC+8)
Publisher:	SPRINGER-VERLAG
Abstract:	In this study, the cellular localization of the inhibitory effect of a natural flavonoid cirsimaritin against formyl-methionyl-leucyl-phenylalanine (fMLP)-induced respiratory burst in rat neutrophils was investigated. Cirsimaritin concentration-dependently inhibited the superoxide anion (O-2(.-)) generation and O-2 consumption (IC50 11.5 +/- 2.2 muM and 17.0 +/- 3.9 muM, respectively) of neutrophils. Cirsimaritin did not reduce, but slightly enhanced the O-2(.-)-generation in phorbol 12-myristate 13-acetate (PMA)-activated or arachidonic acid-stimulated NADPH oxidase preparation as well as during the autoxidation of dihydroxyfumaric acid. Cirsimaritin did not elevate cellular cAMP levels, and only partially inhibited the fMLP-induced [Ca2+](i) changes in the presence or absence of extracellular Call. The phosphorylation of protein tyrosine, extracellular signal-regulated protein kinase and Akt caused by fMLP were attenuated by cirsimaritin in a concentration-dependent manner. In contrast, cirsimaritin had no effect on the phosphorylation of p38 mitogen-activated protein kinase. Cirsimaritin produced a concentration-dependent reduction in the formation of phosphatidic acid and phosphatidylethanol, in the presence of ethanol, from fMLP-stimulated neutrophils (IC50 15.1 +/- 6.5 muM and 15.6 +/- 3.4 muM, respectively), but did not affect the phosphatidylethanol formation in response to PMA. Under the similar concentration range, cirsimaritin attenuated the membrane translocation of ARF and Rho A. However, the GTPgammaS-stimulated membrane-associated ARF and Rho in cell lysate were unaffected by cirsimaritin. Collectively, these results indicate that the inhibition of fMLP-induced respiratory burst by cirsimaritin in rat neutrophils is likely mainly through the blockade of phospholipase D signaling pathwday.
Relation:	RHEUMATOLOGY INTERNATIONAL 22(5):178-181
Appears in Collections:	[China Medical University Hospital] Jurnal articles

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