Comparison of cyfra 21-1 and squamous cell carcinoma antigen in detecting nasopharyngeal carcinoma

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Title:	Comparison of cyfra 21-1 and squamous cell carcinoma antigen in detecting nasopharyngeal carcinoma
Authors:	Lee, JK;Hsieh, JF;Tsai, SC;Ho, YJ;Sun, SS;Kao, CH
Contributors:	附設醫院核子醫學部;China Med Coll Hosp, Dept Nucl Med, Taichung 400, Taiwan;Jen Ai Hosp, Dept Radiol, Taichung, Taiwan;Taichung Vet Gen Hosp, Dept Nucl Med, Taichung, Taiwan;Chi Mei Fdn Hosp, Dept Nucl Med, Tainan, Taiwan;Show Chwan Mem Hosp, Dept Nucl Med, Changhua, Taiwan
Date:	2001
Issue Date:	2010-09-24 14:36:39 (UTC+8)
Publisher:	ANNALS PUBL CO
Abstract:	To evaluate whether or not (-)-menthol affects arylamine N-acetyltransferase (NAT) activity, we selected human liver tumor cell tine (J 5) for examination. By using high performance liquid chromatography, NAT activity for acetylation of 2-aminofluorene (AF) was determined. (-)Menthol displayed a dose-dependent inhibition to cytosolic NAT activity. Time-course experiments showed that NAT activity measured from intact human liver tumor cells was inhibited by (-)-menthol for up to 24 hrs. But in human liver tumor intact cells, the low doses (0.0032 and 0.032 mM) of (-)-menthol promoted the NAT activity and the high doses (3.2 and 32 mM) of (-)-menthol inhibited NAT activity and the 0.32 mM (-)-menthol did not show any significant differences between control and (-)-menthol treated groups. Using standard steady-state kinetic analysis, it was demonstrated that (-)-menthol was a possible uncompetitive inhibitor (decrease Km and Vmax) to NAT activity in cytosols. This report is the first demonstration which showed (-)-menthol affect on human liver tumor cells NAT activity.
Relation:	ANNALS OF OTOLOGY RHINOLOGY AND LARYNGOLOGY 110(8):775-778
Appears in Collections:	[China Medical University Hospital] Jurnal articles

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