Bone mineral density in young female Chinese dancers

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Title:	Bone mineral density in young female Chinese dancers
Authors:	Tsai, SC;Hsu, HC;Fong, YC;Chiu, CC;Kao, A;Lee, CC
Contributors:	附設醫院核子醫學部;Kao, A, China Med Coll Hosp, Dept Nucl Med, 2 Yuh Der Rd, Taichung 404, Taiwan
Date:	2001
Issue Date:	2010-09-24 14:36:13 (UTC+8)
Publisher:	SPRINGER-VERLAG
Abstract:	A protocol for de novo regeneration and rapid micropropagation of Scrophularia yoshimurae (Scrophulariaceae) has been developed. Multiple shoot development was achieved by culturing the shoot-tip, leaf-base, stem-node and stem-internode explants on Murashige and Skoog (MS) medium supplemented with 4.44 muM N-6-benzyladenine (BA) and 1.07 muM alpha -naphthaleneacetic acid (NAA). Stem-node and shoot-tip explants showed the highest response (100%) followed by stem-internode (74.4%) and leaf-base (7.7%) explants. The shoots were multiplied by subculturing on the same medium used for shoot induction. Shoots were rooted on growth regulator-free MS basal medium and the plantlets were transplanted to soil and acclimatized in the growth chamber. The content of harpagoside, a quantitatively predominant iridoid glycoside, in different plant material was determined by high performance liquid chromatography (HPLC). The analysis revealed that the content of harpagoside in the aerial and underground parts of S. yoshimurae was significantly higher than the marketed crude drug (underground parts of Scrophularia ningpoensis).
Relation:	INTERNATIONAL ORTHOPAEDICS 25(5):283-285
Appears in Collections:	[China Medical University Hospital] Jurnal articles

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