Beta-2-microglobulin (beta M-2) as a tumor marker in nasopharyngeal carcinoma

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题名:	Beta-2-microglobulin (beta M-2) as a tumor marker in nasopharyngeal carcinoma
作者:	Lee, JK;Tsai, SC;Hsieh, JF;Ho, YJ;Sun, SS;Kao, CH
贡献者:	附設醫院核子醫學部;China Med Coll Hosp, Dept Nucl Med, Taichung 400, Taiwan;Show Chwan Mem Hosp, Dept Nucl Med, Changhua, Taiwan;Chi Mei Fdn Hosp, Dept Nucl Med, Tainan, Taiwan;Jen Ai Hosp, Dept Radiol, Taichung, Taiwan;Taichung Vet Gen Hosp, Dept Nucl Med, Taichung, Taiwan
日期:	2000
上传时间:	2010-09-24 14:34:25 (UTC+8)
出版者:	INT INST ANTICANCER RESEARCH
摘要:	An efficient method has been developed for regeneration of complete plants via somatic embryogenesis in Corydalis yanhusuo (Fumariaceae), an important medicinal plant, using tuber-derived callus. Primary callus was induced by culturing mature tuber pieces on Murashige and Skoog's (MS) medium supplemented with 2.0 mg 1(-1) N-6-benzyladenine (BA) and 0.5 mg 1(-1) alpha -naphthaleneacetic acid (NAA) in darkness. Somatic embryos were induced by subculturing the primary callus on MS medium supplemented with 0.5-4.0 mg 1(-1) BA, kinetin, or zeatin, within 2 weeks of culture in light. Embryos with well-developed cotyledonary leaves were transferred in half-strength liquid MS medium supplemented with 1.0 mg 1(-1) zeatin riboside for the development of roots. Converted somatic embryos were cultured on half-strength MS medium supplemented with 6% sucrose, and with 0.5-10.0 mg 1(-1) abscisic acid (ABA), paclobutrazol, or ancymidol, 0.5-5.0 mg 1(-1) GA(3) and 15-100 mg 1(-1) polyethylene glycol (PEG) 4000 for further development of plantlets and in vitro tuber formation. The development of somatic embryos over the surface of tuber and/or cotyledonary leaf base region of the converted primary somatic embryo was observed. Before ex vitro establishment of somatic embryo-derived plants, plants with well-developed tubers were cultured on half-strength MS medium with 2% sucrose and 0.1 mg 1(-1) GA(3) for 3 weeks. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
關聯:	ANTICANCER RESEARCH 20(6C):4765-4768
显示于类别:	[台中附設醫院] 期刊論文

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