Monitoring bone loss using ELISA and surface plasmon resonance (SPR) technology

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Please use this identifier to cite or link to this item: http://ir.cmu.edu.tw/ir/handle/310903500/29423

Title:	Monitoring bone loss using ELISA and surface plasmon resonance (SPR) technology
Authors:	Lung, FDT;Chen, HY;Lin, HT
Contributors:	健康照護學院營養系;China Med Coll, Dept Nutr, Taichung 404, Taiwan;China Med Coll Hosp, Dept Obstet & Gynecol, Taichung, Taiwan
Date:	2003
Issue Date:	2010-09-24 14:34:16 (UTC+8)
Publisher:	BENTHAM SCIENCE PUBL LTD
Abstract:	Background Angiogenesis is an important process in tissue development and wound healing. The Tie-2 receptor tyrosine kinases and ligands, angiopoietin (Ang)-1 and -2 have been postulated to play key roles in vascular development. The purpose of this study was to evaluate the expression of Tie-2 and Ang-1 and -2 in an acetic acid-induced gastric ulcer healing process in rats. Methods. Gastric specimens were obtained at 0 (control), 1, 3, 5, 7, and 14 days after ulcer induction for reverse transcription polymerase chain reaction (RTPCR), Western blot analysis, and immunohistochemical analysis. Results. Expression of Tie-2 and Ang-1 and -2 mRNAs was detected in normal gastric tissue and ulcerative tissues by RT-PCR. Western blot analysis revealed that Tie-2 expression reached a maximum on the third to fifth days. Expression of Ang-1 and -2 peaked on the first day. Ang-1 expression gradually became weaker in 2 weeks, whereas Ang-2 expression returned to normal in a few days. Immunohistochemically, Tie-2 was expressed constitutively in the endothelial cells of pre-existing vessels of the gastric wall, and Tie-2 expression was increased in the new capillaries of the ulcer base. Conclusions. These findings suggest that Tie-2 and Ang-1 and -2 play an important role in angiogenesis in the early phase of ulcer healing.
Relation:	PROTEIN AND PEPTIDE LETTERS 10(3):313-319
Appears in Collections:	[Graduate Institute of Nutrition ] Journal articles

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