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請使用永久網址來引用或連結此文件:
http://ir.cmu.edu.tw/ir/handle/310903500/29403
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| 題名: | Efficacy and safety of Monascus purpureus Went rice in subjects with hyperlipidemia |
| 作者: | Lin, CC;Li, TC;Lai, MM |
| 貢獻者: | 附設醫院家醫科;China Med Univ Hosp, Dept Family Med, Taichung 40447, Taiwan;China Med Univ, Dept Inst Chinese Med & Publ Hlth, Taichung 40402, Taiwan |
| 日期: | 2005 |
| 上傳時間: | 2010-09-24 14:33:54 (UTC+8) |
| 出版者: | BIO SCIENTIFICA LTD |
| 摘要: | The aim of this study was to assess the efficacy human mesenchymal stem cells (hMSC) for targeting microscopic tumors and suicide gene or cytokine gene therapy. Immunodeficient mice were transplanted s.c. with human colon cancer cells of HT-29 Inv2 or CCS line, and 3 to 4 days later, i.v. with "tracer" hMSCs expressing herpes simplex virus type 1 thymidine kinase (HSV1-TK) and enhanced green fluorescent protein (EGFP) reporter genes. Subsequently, these tumors were examined for specificity and magnitude of HSV1-TK+, EGFP(+) stem cell engraftment and proliferation in tumor stroma by in vivo positron emission tomography (PET) with F-18-labeled 9-(4-fluoro-3-hydroxymethylbutyl)-guanine ([F-18]-FHBG). In vivo PET images of tumors growing for 4 weeks showed the presence of HSV1-TK+ tumor stroma with an average of 0.36 +/- 0.24% ID/g [F-18]-FHBG accumulation. In vivo imaging results were validated by in situ correlative histochemical, immunofluorescent, and cytometric analyses, which revealed EGFP expression in vWF(+) and CD31(+) endothelial cells of capillaries and larger blood vessels, in germinal layer of dermis and hair follicles proximal to the s.c. tumor site. These differentiated HSV1-TK+, GFP(+) endothelial cells had limited proliferative capacity and a short life span of < 2 weeks in tumor fragments transplanted into secondary hosts. We conclude that hMSCs can target microscopic tumors, subsequently proliferate and differentiate, and contribute to formation of a significant portion of tumor stroma. PET imaging should facilitate clinical translation of stem cell-based anticancer gene therapeutic approaches by providing the means for in vivo noninvasive whole-body monitoring of trafficking, tumor targeting, and proliferation of HSV1-tk-expressing "tracer" hMSCs in tumor stroma. |
| 關聯: | EUROPEAN JOURNAL OF ENDOCRINOLOGY 153(5):679-686 |
| 顯示於類別: | [台中附設醫院] 期刊論文
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