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請使用永久網址來引用或連結此文件:
http://ir.cmu.edu.tw/ir/handle/310903500/29383
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| 題名: | Humic acid-induced echinocyte transformation in human erythrocytes: Characterization of morphological chances and determination of the mechanism underlying damage |
| 作者: | Hseu, YC;Lu, FJ;Engelking, LR;Chen, CL;Chen, YH;Yang, HL |
| 貢獻者: | 健康照護學院營養系;China Med Coll, Dept Nutr, Taichung 40421, Taiwan;Eastern Coll, Dept Food Sci & Technol, Kaohsiung, Taiwan;Natl Taiwan Univ, Coll Med, Dept Biochem, Taipei 10018, Taiwan;Tufts Vet Sch, Dept Biomed Sci, North Grafton, MA USA;China Med Coll, Dept Occupat Safety & Hlth, Taichung, Taiwan |
| 日期: | 2000 |
| 上傳時間: | 2010-09-24 14:33:32 (UTC+8) |
| 出版者: | TAYLOR & FRANCIS LTD |
| 摘要: | OBJECTIVE: To compare empty zona pellucidae (ZP) and human of mouse oocytes for sperm cryopreservation. STUDY DESIGN: ZP was prepared by evacuating the cytoplasm of human or mouse oocytes. The evacuated ZP were injected with spermatozoa from patients with severe oligoasthenozoospermia from normally fertile men. After a freezing-thawing procedure, the spermatozoa were aspirated outside the ZP. According to the different origins of ZP and sperm, the spermatozoa number per ZP, motile sperm number before freezing and after thawing lost sperm number per ZP after freezing and sperm recovery rate were compared. RESULTS: Spermatozoa number, motile sperm number before freezing and after thawing, lost sperm number per ZP and sperm recovery rate in all groups were comparable. The total mean motile sperm number before freezing and after thawing, mean lost sperm number after thawing and sperm recovery rate were 15.1%, 12%, 1.6% and 80%, respectively. CONCLUSION: ZP is an ideal vehicle for cryopreservation of sperm collected from patients with severe oligoasthenozoospermia. There were no differences between human and mouse ZP for sperm storage. |
| 關聯: | JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES 60(3):215-230 |
| 顯示於類別: | [營養學系暨碩士班 ] 期刊論文
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