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http://ir.cmu.edu.tw/ir/handle/310903500/29359
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| 題名: | Effect of garlic oil on hepatic arachidonic acid content and immune response in rats |
| 作者: | Liu, CT;Chen, HW;Sheen, LY;Kung, YL;Chen, PCH;Lii, CK |
| 貢獻者: | 健康照護學院營養系;Chung Shan Med Coll, Dept Nutr, Taichung, Taiwan;Chung Shan Med Coll, Dept Pathol, Taichung, Taiwan;China Med Coll, Dept Nutr, Taichung, Taiwan |
| 日期: | 1998 |
| 上傳時間: | 2010-09-24 14:33:08 (UTC+8) |
| 出版者: | AMER CHEMICAL SOC |
| 摘要: | The inhibitory effect of 2-phenyl-4-quinolone (YT-1) on respiratory burst in rat neutrophils was investigated, and the underlying mechanism of action was assessed. YT-1 caused a concentration-dependent inhibition of the rate of O-2(.-) release from rat neutrophils in response to formylmethionyl-leucyl-phenylalanine (fMLP), but not to phorbol 12-myristate 13-acetate (PMA), with an IC50 value of 60.7 +/- 8.2 mu M. A comparable effect was also demonstrated in the inhibition of O-2 consumption. Unlike superoxide dismutase, YT-1 had no effect on O-2(.-) generation in the xanthine-xanthine oxidase system and during dihydroxyfumaric acid autoxidation. The fMLP-induced inositol trisphosphate (IP3) formation was unaffected by YT-1. In addition, YT-1 did not affect the initial spike of [Ca2+](i), but it accelerated the rate of [Ca2+](i) decline in cells in response to fMLP. YT-1 was found to have little effect on the activity of neutrophil cytosolic protein kinase C (PKC). YT-1 increased the cellular cyclic AMP level, while having no effect on the cyclic GMP level. In addition, YT-1 increased neutrophil cytosolic protein kinase A (PKA) activity, but had no direct effect on the enzyme activity of pure porcine heart PKA. When neutrophils were treated with (8R,9S,11S)-(-)-9-hydroxy-9-hexoxycarbonyl-8-methyl-2,3,9,10-tetrahydro-8,11-epoxy-1H,8H,11H-2,7b,11a-triazadibenzo[a,g]cycloocta[cde]trinden-1-one, (KT 5720), a PKA inhibitor, the inhibition of O-2(.-) generation by YT-1, as well as by prostaglandin E-1 (PGE(1)) and dibutyryl cyclic AMP, was attenuated effectively. YT-1 did not activate the adenylate cyclase associated with neutrophil particulate fraction but inhibited the cytosolic phosphodiesterase (PDE) activity in a concentration-dependent manner. Neutrophils treated with YT-1 had a more pronounced increase in cellular cyclic AMP level by PGE,. Moreover, the ability of PGE, to inhibit the respiratory burst in neutrophils was greatly enhanced by YT-1. These results suggest that the increase in cellular cyclic AMP levels by YT-1 through the inhibition of PDE (probably PDE4 isoenzyme) activity is involved in its inhibition of fMLP-induced respiratory burst in rat neutrophils. BIOCHEM PHARMACOL 56;11:1505-1514, 1998. (C) 1998 Elsevier Science Inc. |
| 關聯: | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 46(11):4642-4647 |
| 顯示於類別: | [營養學系暨碩士班 ] 期刊論文
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