Urokinase gene 3'-UTR T/C polymorphism is not associated with bladder cancer

中國醫藥大學機構典藏 China Medical University Repository, Taiwan > 台中附設醫院 > 期刊論文 > Item 310903500/29325

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题名:	Urokinase gene 3'-UTR T/C polymorphism is not associated with bladder cancer
作者:	Wu, HC;Chang, CH;Chen, WC;Chen, HY;Tsai, FJ
贡献者:	附設醫院泌尿部;China Med Univ Hosp, China Med Coll Hosp, Dept Urol, Taichung 404, Taiwan;China Med Univ Hosp, Dept Med Genet, Taichung, Taiwan;China Med Univ Hosp, Dept Obstet & Gynecol, Taichung, Taiwan;China Med Univ Hosp, Dept Pediat, Taichung, Taiwan
日期:	2004
上传时间:	2010-09-24 14:32:02 (UTC+8)
出版者:	SOC BRASIL GENETICA
摘要:	Leukemia inhibitory factor (LIF) is an essential factor for implantation and establishment of pregnancy. However, its role in the development of preimplantation embryos remains controversial. In this study, changes in preimplantation embryos were determined after microinjection of LIF antisense oligonucleoticle at the two-pronucleus stage. Although no significant differences were found in the percentages between the untreated group and the 0.25-fmol-treated group, the 0.5- or 1.0-fmol-treated groups had significantly lower percentages of embryos developed to the morula or blastocyst stage and the 2.0-fmol-treated group had significantly lower percentages of embryos developed to the four-cell, morula, or blastocyst stage. No embryos developed to the four-cell stage in the 4.0-fmol-treated group. Moreover, there was a decreasing trend in the levels of LIF immunoactivity with the increasing amount of LIF antisense oligonucleoticle injected. The diameter of blastocysts in the 2.0-fmol-treated group was significantly smaller than that in the untreated group. The blastocysts in this group had significantly lower numbers of blas-tomeres and cells in the inner cell mass (ICM) or trophectoderm (TE) and ICM:TE ratio. The 1.0- or 2.0-fmol-treated groups had significantly lower implantation rates than their corresponding control groups. In the 2.0-fmol groups with supplementing exogenous LIF, significantly lower percentages were also observed in the four-cell, morula, and blastocyst stages. However, blastocysts treated with 50 ng/ml LIF had a significantly higher percentage than those in the LIF gene-impaired group without LIF supplement. These results indicate that LIF is a critical factor for the normal development of embryos at the preimplantation stages.
關聯:	GENETICS AND MOLECULAR BIOLOGY 27(1):15-16
显示于类别:	[台中附設醫院] 期刊論文

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