Comparison of chemotherapy response with P-glycoprotein, multidrug resistance-related protein-1, and lung resistance-related protein expression in untreated small cell lung cancer

中國醫藥大學機構典藏 China Medical University Repository, Taiwan > 台中附設醫院 > 期刊論文 > Item 310903500/29266

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题名:	Comparison of chemotherapy response with P-glycoprotein, multidrug resistance-related protein-1, and lung resistance-related protein expression in untreated small cell lung cancer
作者:	Yeh, JJ;Hsu, NY;Hsu, WH;Tsai, CH;Lin, CC;Liang, JA
贡献者:	附設醫院放射腫瘤科;China Med Univ Hosp, Dept Radiat Therapy & Oncol, Taichung 404, Taiwan;Ping Tung Christian Hosp, Dept Thorac Med, Pingtung, Taiwan;Mei Ho Inst Technol, Pingtung, Taiwan;China Med Univ Hosp, Div Chest Surg, Taichung, Taiwan;China Med Univ Hosp, Div Pulm & Crit Care Med, Taichung, Taiwan;Taichung Healthcare & Management Univ, Grad Inst Bioinformat, Taichung, Taiwan
日期:	2005
上传时间:	2010-09-24 14:29:59 (UTC+8)
出版者:	SPRINGER
摘要:	This paper introduces a newly developed method of applying liquid chromatography- tandem mass spectrometry (LC/MS/MS) to the analysis of aristolochic acids (AAs) in Chinese herbal (medicinal) preparations. Sensitive and highly accurate readings were obtained for this study using both a photodiode array (PDA) detector and tandem mass spectrometer. The following optimized conditions for LC/MS/MS were set for this study: Separation was accomplished using a reverse phase C18 column (2.1 x 150 mm, 5 pm). The mobile phase comprised 35% acetonitrile and a 65% aqueous solution containing 0.1% formic acid and 0.1% ammonium acetate at a flow rate of 0.3 mL/min with a split ratio of 1:1 into the PDA detector and the tandem mass spectrometer. MS/MS qualitative analysis was performed at 3.0 kV capillary voltage, with a collision energy level for aristolochic acid I (AA-I) of 10 eV and for aristolochic acid II (AA-II) of 12eV. The electrospray ionization source was operated in the positive mode. AA-I [M + NH4](+) ions at m/z 359 and AA-II [M + NH4](+) ions at m/z 329 were selected as precursor ions for daughter ion scanning. The characteristic daughter ion mass spectra for AA-I and AA-II were generated and studied carefully. Quantification of results was done using the multiple reaction monitoring (MRM) method. The [M + NH4](+) and [(M + NH4)- NH3-CO2](+) ions of both AA-I and AA-II were selected as precursor and daughter ions for the MRM analysis. MS/MS detection limits were defined as 2.0 ng/mL of AA-I, and 2.8 ng/mL of AA-II. The linear regression correlation coefficients of the calibration cure were 0.9992 of AA-I within the range of 0.02 similar to 16.00 Pg/mL and 0.9988 of AA-II within the range of 0.028 similar to 22.40 mu g/mL. Relative standard deviations of 0.73% and 10.44% for AA-I, and 1.38% and 6.10% for AA-II were determined for the intraday and interday tests. Recoveries for five differing concentrations of AA-I ranged from 99.0% to 106.9% and, for five differing concentrations of AA-II, ranged from 92.0% to 104.5%. Levels of AA-I and AA-II detected in the 12 commercial Chinese medicinal preparation samples ranged from 11.1 to 3376.0 pg/g and from 5.4 to 725.4 pg/g, respectively.
關聯:	LUNG 183(3):177-183
显示于类别:	[台中附設醫院] 期刊論文

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