Safety monitoring of cardiac and hepatic systems in beta-thalassemia patients with chelating treatment in Taiwan

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Title:	Safety monitoring of cardiac and hepatic systems in beta-thalassemia patients with chelating treatment in Taiwan
Authors:	Peng, CT;Chow, KC;Chen, JH;Chiang, YP;Lin, TY;Tsai, CH
Contributors:	附設醫院兒科部;China Med Coll Hosp, Dept Paediat, Taichung 404, Taiwan;China Med Coll Hosp, Dept Med Res, Taichung 404, Taiwan;China Med Coll Hosp, Dept Radiol, Taichung 404, Taiwan;China Med Coll Hosp, Dept Pathol, Taichung 404, Taiwan;China Med Coll Hosp, Inst Med Sci, Taichung 404, Taiwan
Date:	2003
Issue Date:	2010-09-24 14:27:01 (UTC+8)
Publisher:	BLACKWELL MUNKSGAARD
Abstract:	Current serological tests for melioidosis, using impure or uncharacterized cell antigens from Burkholderia pseudomallei, have problems in detection sensitivity and specificity. Therefore, we designed and expressed the recombinant flagellin (truncated at both the N- and C-terminal ends), and used the antigen to develop an indirect enzyme-linked immunosorbent assay (ELISA) to diagnose melioidosis. Comparison of the immunoreactivities of the full-length and truncated flagellins reveals that the truncated flagellin performed much better in detection specificity and sensitivity. Only the full-length flagellin was recognized by other bacterial causing septicemia and gave a false-positive result in Western analysis, indicating that the cross-reactive epitopes were located on the more highly conserved N- and C-terminal regions of flagellin. The indirect ELISA using recombinant truncated flagellin as the antigen achieved 93.8% sensitivity and 96.3% specificity and offered a more efficient serodiagnosis of melioidosis.
Relation:	EUROPEAN JOURNAL OF HAEMATOLOGY 70(6):392-397
Appears in Collections:	[China Medical University Hospital] Jurnal articles

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