Shikonin-induced Apoptosis Involves Caspase-3 Activity in a Human Bladder Cancer Cell Line (T24)

中國醫藥大學機構典藏 China Medical University Repository, Taiwan > 中醫學院 > 中醫學系暨碩博班 > 期刊論文 > Item 310903500/2917

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.cmu.edu.tw/ir/handle/310903500/2917

题名:	Shikonin-induced Apoptosis Involves Caspase-3 Activity in a Human Bladder Cancer Cell Line (T24)
作者:	葉進仲(Jin-Jung Ye);郭秀滿;李德茂(Te-Mao Li);林景彬(Jing-Pin Lin);游富順;(Hsu-Feng Lu);鍾景光(Jing-Gung Chung);楊家欣(Yang Jai-Sing)
贡献者:	中醫學院中醫學系學士班中醫內科學科;中國附醫泌尿部
关键词:	Shikonin;apoptosis;caspase-3;human bladder cancer;Western blotting;caspase-activated DNase
日期:	2007-12
上传时间:	2009-08-20 17:57:40 (UTC+8)
摘要:	Apoptosis is a process that leads to programmed cell death and also a therapeutic target of cancer. In this study, potential apoptotic effects of shikonin on human bladder cancer cells (T24) in vitro were evaluated. Apoptosis induction, cell viability and morphological changes were investigated and caspase-3 and -9 activity was determined by flow cytometric assay and reverse transcription-polymerase chain reaction. The results showed marked differences in G0/G1 cell cycle arrest and cell death of the T24 cells between shikonin treated and untreated groups. Within 72 hours of treatment, shikonin influenced the cyclin dependent kinase (CDK) and cyclin activity by increasing p21 and decreasing cyclin E, CDK2 and CDK4 protein levels. A marked increase was found in apoptosis induction when the T24 cells were treated with shikonin compared to the untreated group, also confirmed by flow cytometry assay. Shikonin also promoted caspase-3 activity, which led to the induction of caspase-activated DNase (CAD) and cleavage poly(ADP-ribose)polymerase. Furthermore, the shikonin-induced apoptosis of the T24 cells was markedly blocked by the broad-spectrum caspase inhibitor, z-VAD-fmk. Shikonin may be a potential agent for the treatment of bladder transitional cell carcinoma since it induces apoptosis through the activation of caspase-3 activity in T24 human bladder cancer cells.
關聯:	IN VIVO 21(6):1011~1020
显示于类别:	[中醫學系暨碩博班] 期刊論文

文件中的档案:

档案	大小	格式	浏览次数
	0Kb	Unknown	389	检视/开启

在CMUR中所有的数据项都受到原著作权保护.

TAIR相关文章

数据加载中.....