Two common mutations 934C to G and 937C to G of fibroblast growth factor receptor 2 (FGFR2) gene in Chinese patients with apert syndrome

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Title:	Two common mutations 934C to G and 937C to G of fibroblast growth factor receptor 2 (FGFR2) gene in Chinese patients with apert syndrome
Authors:	Tsai, FJ;Hwu, WL;Lin, SP;Chang, JG;Wang, TR;Tsai, CH
Contributors:	附設醫院兒科部;China Med Coll Hosp, Dept Pediat, Taipei, Taiwan;Natl Taiwan Univ Hosp, Dept Pediat & Med Genet, Taipei, Taiwan;Mackay Mem Hosp, Dept Pediat, Taipei, Taiwan;Taipei Municipal Jen Ai Hosp, Dept Mol Med, Taipei, Taiwan
Date:	1998
Issue Date:	2010-09-24 14:25:18 (UTC+8)
Publisher:	WILEY-LISS
Abstract:	In order to investigate the mechanism of long-term immunity and the effect of protective immunity induced by DNA vaccination, we constructed the expression plasmid containing a pseudorabies virus (PRV) gD gene encoding an envelope glycoprotein. Intramuscular vaccination of mice with the plasmid DNA induced a strong antibody response which lasted for one year after final vaccination. An IgM to IgG class switch occurred, indicating helper T-lymphocyte activity. We further analyzed the persistence and expression of gD gene by polymerase chain reaction and reverse transcriptase polymerase chain reaction. The results showed that gD gene was present and expressed in the muscle cell up to one year after final booster injection. Furthermore, mice vaccinated with the plasmid DNA were protected against a subsequent lethal challenge with PRV. Therefore, the DNA vaccination does induce a protective immunity and long-term antibody response against PRV, which could be maintained by persistent expression of gD gene in muscle cells.
Relation:	HUMAN MUTATION ():S18-S19
Appears in Collections:	[China Medical University Hospital] Jurnal articles

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