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資料載入中.....
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請使用永久網址來引用或連結此文件:
http://ir.cmu.edu.tw/ir/handle/310903500/29172
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| 題名: | Diphtheria, tetanus and whole cell pertussis vaccine combined with hepatitis B vaccines: A comparison of two doses (10 mu g and 5 mu g) |
| 作者: | Chiu, HH;Huang, LM;Lee, PI;Safary, A;Lee, CY |
| 貢獻者: | 附設醫院兒科部;Natl Taiwan Univ Hosp, Dept Pediat, Taipei 10016, Taiwan;China Med Coll Hosp, Dept Pediat, Taichung, Taiwan;SmithKline Beecham Biol, Rixensart, Belgium |
| 日期: | 1998 |
| 上傳時間: | 2010-09-24 14:25:15 (UTC+8) |
| 出版者: | LIPPINCOTT WILLIAMS & WILKINS |
| 摘要: | The pseudorabies virus (PRV) DNase gene has previously been mapped within the PRV genome. To characterize further the enzymic properties of PRV DNase, this enzyme was expressed in Escherichia coli with the use of a pET expression vector. The protein was purified to homogeneity and assayed for nuclease activity in vitro. Recombinant PRV DNase exhibited an alkaline pH preference and an absolute requirement for Mg2+ ions that could not be replaced by Ca2+ and Na+ ions. Further studies showed that PRV DNase exhibited endonuclease, 5'-exonuclease and 3'-exonuclease activities in both single-stranded and double-stranded DNA. This activity occurred randomly and no significant base preference was demonstrated, The multiple biochemical activities of PRV DNase are similar to the activities of Neurospora crassa endo-exonuclease and E. coli RecBCD, two additional enzymes that are involved in recombination. Taken together, the similarity of action between N. crassa endo-exonuclease, E. coli RecBCD, and PRV DNase suggests that PRV DNase might have a role in the process of recombination that occurs during PRV infection. |
| 關聯: | PEDIATRIC INFECTIOUS DISEASE JOURNAL 17(3):206-211 |
| 顯示於類別: | [台中附設醫院] 期刊論文
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