Characterization of spiculation on ultrasound lesions

中國醫藥大學機構典藏 China Medical University Repository, Taiwan > 台中附設醫院 > 期刊論文 > Item 310903500/29132

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题名:	Characterization of spiculation on ultrasound lesions
作者:	Huang, SF;Chang, RF;Chen, DR;Moon, WK
贡献者:	附設醫院外科部;Natl Chung Cheng Univ, Dept Comp Sci & Informat Engn, Chiayi 621, Taiwan;China Med Coll & Hosp, Dept Gen Surg, Taichung 404, Taiwan;Seoul Natl Univ Hosp, Dept Diagnost Radiol, Coll Med, Seoul 110744, South Korea
日期:	2004
上传时间:	2010-09-24 14:23:13 (UTC+8)
出版者:	IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
摘要:	Severe acute respiratory syndrome (SARS) is an emerging infectious disease associated with a novel coronavirus and causing worldwide outbreaks. SARS coronavirus (SARS-CoV) is an enveloped RNA virus, which contains several structural proteins. Among these proteins, spike (S) protein is responsible for binding to specific cellular receptors and is a major antigenic determinant, which induces neutralizing antibody. In order to analyze the antigenicity and receptor-binding ability of SARS-CoV S protein, we expressed the S protein in Escherichia coli using a pET expression vector. After the isopropyl-p-D-thiogalactoside induction, S protein was expressed in the soluble form and purified by nickel-affinity chromatography to homogeneity. The amount of S protein recovered was 0.2-0.3 mg/100 ml bacterial culture. The S protein was recognized by sera from SARS patients by ELISA and Western blot, which indicated that recombinant S protein retained its antigenicity. By biotinylated ELISA and Western blot using biotin-labeled S protein as the probe, we identified 130-kDa and 140-kDa proteins in Vero cells that might be the cellular receptors responsible for SARS-CoV infection. Taken together, these results suggested that recombinant S protein exhibited the antigenicity and receptor-binding ability, and it could be a good candidate for further developing SARS vaccine and anti-SARS therapy. (C) 2003 Elsevier Inc. All rights reserved.
關聯:	IEEE TRANSACTIONS ON MEDICAL IMAGING 23(1):111-121
显示于类别:	[台中附設醫院] 期刊論文

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