Characterization of spiculation on ultrasound lesions

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Title:	Characterization of spiculation on ultrasound lesions
Authors:	Huang, SF;Chang, RF;Chen, DR;Moon, WK
Contributors:	附設醫院外科部;Natl Chung Cheng Univ, Dept Comp Sci & Informat Engn, Chiayi 621, Taiwan;China Med Coll & Hosp, Dept Gen Surg, Taichung 404, Taiwan;Seoul Natl Univ Hosp, Dept Diagnost Radiol, Coll Med, Seoul 110744, South Korea
Date:	2004
Issue Date:	2010-09-24 14:23:13 (UTC+8)
Publisher:	IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
Abstract:	Severe acute respiratory syndrome (SARS) is an emerging infectious disease associated with a novel coronavirus and causing worldwide outbreaks. SARS coronavirus (SARS-CoV) is an enveloped RNA virus, which contains several structural proteins. Among these proteins, spike (S) protein is responsible for binding to specific cellular receptors and is a major antigenic determinant, which induces neutralizing antibody. In order to analyze the antigenicity and receptor-binding ability of SARS-CoV S protein, we expressed the S protein in Escherichia coli using a pET expression vector. After the isopropyl-p-D-thiogalactoside induction, S protein was expressed in the soluble form and purified by nickel-affinity chromatography to homogeneity. The amount of S protein recovered was 0.2-0.3 mg/100 ml bacterial culture. The S protein was recognized by sera from SARS patients by ELISA and Western blot, which indicated that recombinant S protein retained its antigenicity. By biotinylated ELISA and Western blot using biotin-labeled S protein as the probe, we identified 130-kDa and 140-kDa proteins in Vero cells that might be the cellular receptors responsible for SARS-CoV infection. Taken together, these results suggested that recombinant S protein exhibited the antigenicity and receptor-binding ability, and it could be a good candidate for further developing SARS vaccine and anti-SARS therapy. (C) 2003 Elsevier Inc. All rights reserved.
Relation:	IEEE TRANSACTIONS ON MEDICAL IMAGING 23(1):111-121
Appears in Collections:	[China Medical University Hospital] Jurnal articles

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