中國醫藥大學機構典藏 China Medical University Repository, Taiwan:Item 310903500/29062
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    题名: The use of dual phase (TI)-T-201 SPECT for differentiating pulmonary malignancies from benign lesions
    作者: Yu, YH;Hsu, WH;Hsu, NY;Lin, TY;Hsia, TC;Sun, SS;Kao, CH
    贡献者: 附設醫院內科部呼吸治療科;China Med Univ Hosp, Dept Med, Div Pulm & Crit Care Med, Taichung, Taiwan;China Med Univ Hosp, Dept Surg, Div Thorac Surg, Taichung, Taiwan;China Med Univ Hosp, Dept Pathol, Taichung, Taiwan;China Med Univ Hosp, Dept Nucl Med, Taichung, Taiwan
    日期: 2004
    上传时间: 2010-09-24 14:20:16 (UTC+8)
    出版者: OXFORD UNIV PRESS
    摘要: Shi-hu (Dendrobium spp. or Dendrobii Herba) is one of the important traditional Chinese medicines. The commercially available crude drug in the traditional medicine market is composed mainly of three species: Dendrobium tosaense, D. nobile, and D. moniliforme. An efficient method of propagation has been developed via asymbiotic germination of seeds in vitro for the medicinally important D. tosaense. Seeds from capsules of D. tosaense collected 8-14 wk after artificial pollination germinated after being cultured on full-strength or half-strength Murashige and Skoog (MS) medium devoid of plant growth regulators and with 3% sucrose. Germination of seeds varied with the medium type and seed maturity. Germinated seedlings after transfer to MS medium with 1.5% sucrose and 8% banana homogenate or potato juice or coconut water and 20 wk of incubation developed into healthy plantlets. Well-developed plantlets were transplanted to moss or moss and tree fern or tree fern as substrates in plastic trays and transferred to a greenhouse for hardening. All plants survived, attained maturity, and developed normal flower and capsule after one and a half years. This protocol of successful plant regeneration by asymbiotic seed germination should permit rapid propagation and conservation of this medicinally important Dendrobium species.
    關聯: JAPANESE JOURNAL OF CLINICAL ONCOLOGY 34(8):445-451
    显示于类别:[台中附設醫院] 期刊論文

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