Association between Fibrillin-1 gene exon 15 and 27 Polymorphisms and risk of mitral valve prolapse

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Title:	Association between Fibrillin-1 gene exon 15 and 27 Polymorphisms and risk of mitral valve prolapse
Authors:	Chou, HT;Shi, YR;Hsu, Y;Tsai, FJ
Contributors:	附設醫院內科部心臟科;China Med Coll Hosp, Dept Med, Div Cardiol, Taichung 404, Taiwan;China Med Coll Hosp, Dept Pediat Med Res & Med Genet, Taichung 404, Taiwan
Date:	2003
Issue Date:	2010-09-24 14:19:29 (UTC+8)
Publisher:	I C R PUBLISHERS
Abstract:	The effects of gypenosides on the inhibition of N-acetyltransferase (NAT) activity, AF-DNA adduct formation and NAT gene expression in a human cervix cancer cell line (Ca Ski) were studied. Various concentrations of gypenosides were added to the cytosols or individually to the culture medium of human cervix cancer cells. The NAT activity was determined by high performance liquid chromatography, assaying for the amounts of acetylated 2-aminofluorene (AAF) and non-acetylated 2-aminofluorene (AF). The NAT activity in the human cervix intact cancer cells and cytosols was suppressed by gypenosides in a dose-dependent manner. The results also demonstrated that gene expression (NAT1 mRNA) in human cervix cancer cells was decreased by gypenosides in a dose-dependent manner. The apparent values of Km and Vmax of NAT of human cervix cancer cells were also decreased by gypenosides in cytosols. Gypenosides may act as noncompetitive inhibitors. After the incubation of human cervix cancer cells with 30 or 60 muM AF and with or without 350 mug/ml gypenosides co-treatment, the cells were recovered, DNA was prepared and hydrolyzed to nucleotides; adducted nucleotides were extracted in butanol and AF-DNA adducts were analyzed by HPGC. The results demonstrated that gypenosides decreased the levels of AF-DNA adduct formation in these cells. The NAT PCR and cDNA microarray also demonstrated that gypenosides inhibited NAT mRNA expression in human cervix cancer cells.
Relation:	JOURNAL OF HEART VALVE DISEASE 12(4):475-481
Appears in Collections:	[China Medical University Hospital] Jurnal articles

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