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| 題名: | Interactions of esmolol and adenosine in atrioventricular nodal-dependent supraventricular tachycardia: Implication for the cellular mechanisms of adenosine |
| 作者: | Chang, KC;Lin, YC;Chen, JY;Chou, HT;Hung, JS |
| 貢獻者: | 附設醫院內科部心臟科;China Med Coll Hosp, Dept Med, Div Cardiol, Taichung, Taiwan |
| 日期: | 2002 |
| 上傳時間: | 2010-09-24 14:19:25 (UTC+8) |
| 出版者: | KARGER |
| 摘要: | NCTD is a demethylated form of cantharidin with antitumor properties, which is now in use as a routine anticancer drug against hepatoma. However, there is limited information on the effect of NCTD on human cancer cells. In the present study, NCTD inhibited proliferation, caused mitotic arrest, then progressed to apoptosis within 96 hr in 3 human hepatoma cell lines: HepG2, Hep3B and Huh-7. NCTD treatment (5 mug/ml) enhanced the expression of Cdc25C and p21(Cip1/Waf1), increasing the phosphorylation of these 2 proteins. In addition, NCTD treatment induced an earlier increase in cyclin B1-associated histone H1 kinase activity within 48 hr, but an approximately 70% reduction of both protein level and kinase activity of cyclin B1 was observed at 72 hr. Treatment with NCTD significantly decreased the expression of p53 protein but did not affect the expression of Cdk1 and P27 (Kip1). Moreover, NCTD treatment also increased the phosphorylation of Bcl-2 and Bcl-X-L- but did not affect the expression of Bax or Bad. Bcl-2 phosphorylation appears to inhibit its binding to Bax since less Bax was detected in immunocomplex with Bcl-2 in NCTD-treated HepG2 cells. In addition, NCTD treatment caused activation of caspase-9 and caspase-3, preceding DNA fragmentation and morphologic features of apoptosis. Pretreatment with the broad-spectrum caspase inhibitor z-VAD-fmk markedly inhibited NCTD-induced caspase-3 activity and cell death. These results suggest that phosphorylation of p21 cip1/Waf1 and Cdc25C and biphasic regulation of cyclin B l-associated kinase activity may contribute to NCTD-induced M-phase cell-cycle arrest. Furthermore, the increase of p21Cip1/Waf1 phosphorylation of Bcl-2 and BCl-X-L, activation of caspase-9 and caspase-3 may be the molecular mechanism through which NCTD induces apoptosis. 2002 Wilev-Liss, Inc. |
| 關聯: | CARDIOLOGY 97(3):138-146 |
| 顯示於類別: | [台中附設醫院] 期刊論文
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