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| 題名: | N-acetyltransferase is involved in baicalein-induced N-acetylation of 2-aminofluorene and DNA-2-aminofluorene adduct formation in human leukemia HL-60 cells |
| 作者: | Li, YC;Tyan, YS;Lee, YM;Tsao, TY;Chuang, JY;Kuo, HM;Hsia, TC;Yang, JH;Chung, JG |
| 貢獻者: | 附設醫院內科部;Chungtai Inst Hlth Sci & Technol, Dept Med Technol, Taichung 406, Taiwan;Chung Shan Med Univ Hosp, Dept Radiol, Taichung 402, Taiwan;Chung Shan Med Univ Hosp, Dept Dermatol, Taichung 402, Taiwan;Tungs Taichung Metroharbor Hosp, Dept Pathol, Taichung, Taiwan;China Med Univ Hosp, Dept Internal Med, Taichung, Taiwan;China Med Coll, Sch Med Lab Sci & Biotechnol, Taichung 400, Taiwan;China Med Coll, Dept Parasitol, Taichung 400, Taiwan;China Med Coll, Dept Microbiol, Taichung 400, Taiwan |
| 日期: | 2005 |
| 上傳時間: | 2010-09-24 14:18:58 (UTC+8) |
| 出版者: | INT INST ANTICANCER RESEARCH |
| 摘要: | The present study was undertaken to determine the effect of acetylsalicylic acid acid on the in vitro N-acetyltransferase (NAT) enzyme activity and in vivo acetylation of 2-aminofluorene in laboratory rats. In the in vitro experiments, cytosols of blood, bladder, colon and liver cells, with or without acetylsalicylic acid co-treatment, showed different percentages of 2-aminofluorene acetylation. The data indicated that there was decreased NAT activity associated with increased acetylsalicylic acid in the cytosol reaction. In the in vitro experiments, values of apparent Kin and Vmax decreased by 4% and 21%, respectively, for acetylation of AF in blood NAT, 28% and 31% for acetylation of AF in bladder NAT, 12% and 25% for acetylation of AF in colon NAT, and 50% and 35% for acetylation of AF in liver NAT In the in vivo experiments, pretreatment with acetylsalicylic acid (50 mg/kg) 48 h prior to the administration of 2-aminofluorene (50 mg/kg) resulted in 24% and 28% decreases in the fecal and urinary recovery of N-acetyl-2-aminofluorene and a 26% decrease in the metabolic clearance of 2-aminofluorene to N-acetyl-2-aminofluorene. This is the first demonstration of acetylsalicylic acid (Aspirin) inhibition of arylamine N-acetyltransferase activity showing decreases in the N-acetylation of carcinogens in vivo. |
| 關聯: | IN VIVO 19(2):399-405 |
| 顯示於類別: | [台中附設醫院] 期刊論文
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