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http://ir.cmu.edu.tw/ir/handle/310903500/29013
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| 題名: | Atropine-induced gastric dysrhythmia is not normalized by electroacupuncture |
| 作者: | Chang, CS;Chou, JW;Wu, CY;Chang, YH;Ko, CW;Chen, GH |
| 貢獻者: | 附設醫院內科部;Taichung Vet Gen Hosp, Dept Internal Med, Div Gastroenterol, Taichung 407, Taiwan;Chung Shan Med Univ, Dept Internal Med, Taichung, Taiwan;China Med Coll Hosp, Dept Internal Med, Taichung, Taiwan |
| 日期: | 2002 |
| 上傳時間: | 2010-09-24 14:18:42 (UTC+8) |
| 出版者: | KLUWER ACADEMIC/PLENUM PUBL |
| 摘要: | 2-Benzyloxybenzaldehyde (CCYla) inhibited the formyl-methionyl-leucyl-phenylalanine (RALP)-stimulated phospholipase D (PLD)mediated products, phosphatidic acid (PA) and phosphatidylethanol (PEt) formation in rat neutrophils in a concentration-dependent manner with IC50 values of 15.8 +/- 2.5 and 13.9 +/- 2.0 muM, respectively. The underlying cellular signaling mechanism of CCYla inhibition was investigated. CCYla inhibited the plateau phase but not the initial Ca2+ spike of fMLP-stimulated Ca2+ signal. CCYla did not inhibit the [Ca2+](i) change in Ca2+-free medium in response to fMLP, but inhibited the [Ca2+](i) change by the subsequent addition of Ca2+. In addition, CCYla treatment attenuated the fMLP-induced protein tyrosine phosphorylation. The membrane translocation of ADP-ribosylation factor (ARF) and Rho A proteins in neutrophils stimulated with fMLP was attenuated by CCYla in a concentration-dependent manner. In a cell-free system, neither the membrane association of ARF and Rho A caused by GTPgammaS nor the phorbol myristate acetate-stimulated membrane translocation of Rho A was suppressed significantly by CCYla. These results indicate that the attenuation of protein tyrosine phosphorylation, blockade of Ca2+ entry, and the suppression of ARF and Rho A membrane translocation are probably obligatory for the CCYla inhibition of PLD activity in rat neutrophils in response to fMLP. (C) 2002 Elsevier Science B.V. All rights reserved. |
| 關聯: | DIGESTIVE DISEASES AND SCIENCES 47(11):2466-2472 |
| 顯示於類別: | [台中附設醫院] 期刊論文
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