資料載入中.....
|
請使用永久網址來引用或連結此文件:
http://ir.cmu.edu.tw/ir/handle/310903500/28997
|
| 題名: | CARCINOID-TUMORS OF THE THYMUS |
| 作者: | WANG, DY;CHANG, DB;KUO, SH;YANG, PC;LEE, YC;HSU, HC;LUH, KT |
| 貢獻者: | 附設醫院內科部;NATL TAIWAN UNIV HOSP,DEPT CLIN PATHOL,TAIPEI 100,TAIWAN;CHINA MED COLL HOSP,DEPT INTERNAL MED,TAICHUNG,TAIWAN;NATL TAIWAN UNIV HOSP,DEPT INTERNAL MED,TAIPEI 100,TAIWAN;NATL TAIWAN UNIV HOSP,DEPT THORAC SURG,TAIPEI 100,TAIWAN;NATL TAIWAN UNIV HOSP,DEPT PATHOL,TAIPEI 100,TAIWAN |
| 日期: | 1994 |
| 上傳時間: | 2010-09-24 14:18:13 (UTC+8) |
| 出版者: | BRITISH MED JOURNAL PUBL GROUP |
| 摘要: | The role of TSH receptor antibodies in the pathogenesis of pretibial myxedema is still unclear. This study was designed to determine whether patients with pretibial myxedema had higher serum titers of TSH receptor antibodies, and whether there were TSH and TSH receptor antibody-binding sites on plasma membranes of fibroblasts derived from the skin of pretibial myxedema. If there were, were the binding sites similar to the TSH receptor? The TSH receptor antibodies were determined with radioreceptor assay in 20 normal subjects, 18 hyperthyroid Graves' disease patients without ophthalmopathy, 26 hyperthyroid Graves' disease patients with ophthalmopathy, and 11 patients with pretibial myxedema associated with Graves' ophthalmopathy. TSH and TSH receptor antibody-binding sites were studied on plasma membranes of fibroblasts cultured from the skin of pretibial myxedema with radioreceptor assay. RNA was also extracted from the fibroblasts of pretibial myxedema and reverse transcribed using random primers as the primers for cDNA synthesis. The resulting cDNAs were subjected to amplification by polymerase chain reaction with the use of a set of primers spanning the 5' region (+256/+275 and +616/+635) and the 3' region (+1819/+1838 and +2405/+2424) of the TSH receptor cDNA(+1 transcription start codon). They were further identified by Southern blot hybridization, with the probe spanning the 5' region (+272/+612) and the 3' region (+1908/+2268) of the TSH receptor cDNA (+1 transcription start codon), and sequencing. The results showed that patients with pretibial myxedema had higher titers of TSH receptor antibodies in the serum. TSH and TSH receptor antibody-binding sites were present on plasma membranes of fibroblasts derived from the skin of pretibial myxedema patients and related to the extracellular domain of the TSH receptor. These data suggest a common antigenic site in the skin and in the thyroid as a putative target for TSH receptor antibodies or lymphocytes of Graves' disease. (C) 1994 Academic Press, Inc. |
| 關聯: | THORAX 49(4):357-360 |
| 顯示於類別: | [台中附設醫院] 期刊論文
|
在CMUR中所有的資料項目都受到原著作權保護.
|
