Vesicle calculus caused by migrant intrauterine device

中國醫藥大學機構典藏 China Medical University Repository, Taiwan > 台中附設醫院 > 期刊論文 > Item 310903500/28949

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题名:	Vesicle calculus caused by migrant intrauterine device
作者:	Lu, HF;Chen, JH;Chen, WC;Shen, WC
贡献者:	附設醫院;China Med Coll Hosp, Taichung 404, Taiwan
日期:	1999
上传时间:	2010-09-24 14:16:47 (UTC+8)
出版者:	AMER ROENTGEN RAY SOC
摘要:	A rapid method, utilizing both polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA), was developed for detection of oyster MSX disease. The technique included using Haplosporidium nelsoni pathogen-specific PCR primers (based on ribosomal RNA genes), a Chelex resin (for rapid DNA extraction from oyster mantle tissues), and cloned H. nelsoni rRNA plasmid DNA (for use as a capture probe). Digoxigenin was incorporated into the pathogen-specific PCR products, which were captured by the coated probe in a fast hybridization reaction and then detected by ELISA. The sensitivity of PCR amplification on cloned plasmid DNA was 10 fg for detection by stained agarose gel, and increased to 0.01 fg for ELISA. Positive signals were observed in infected oysters using the PCR-ELISA technique. This method may be applicable to early detection of infection.
關聯:	AMERICAN JOURNAL OF ROENTGENOLOGY 173(2):504-505
显示于类别:	[台中附設醫院] 期刊論文

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