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| 題名: | Epidemiologic relationship between fluoroquinolone-resistant Salmonella enterica serovar choleraesuis strains isolated from humans and pigs in Taiwan (1997 to 2002) |
| 作者: | Chang, CC;Lin, YH;Chang, CF;Yeh, KS;Chiu, CH;Chu, C;Chien, MS;Hsu, YM;Tsai, LS;Chiou, CS |
| 貢獻者: | 生命科學院生科系;Ctr Dis Control, Cent Branch Off, Taichung 408, Taiwan;Natl Chung Hsing Univ, Grad Inst Vet Publ Hlth, Taichung 402, Taiwan;Natl Taiwan Univ, Dept Vet Med, Taipei 106, Taiwan;Taipei Med Univ, Sch Med, Dept Microbiol & Immunol, Taipei 110, Taiwan;Chang Gung Childrens Hosp, Dept Pediat, Div Pediat Infecious Dis, Taoyuan 333, Taiwan;Natl Chiayi Univ, Dept Appl Microbiol, Chiayi 600, Taiwan;Natl Chung Hsing Univ, Grad Inst Vet Pathol, Taichung 402, Taiwan;China Med Univ, Sch Med, Dept Biol Sci & Technol, Taichung 404, Taiwan |
| 日期: | 2005 |
| 上傳時間: | 2010-09-24 14:15:00 (UTC+8) |
| 出版者: | AMER SOC MICROBIOLOGY
American Society for Microbiology |
| 摘要: | Individuals can be classified into rapid or slow acetylators based on the N-acetyltransferase (NAT) activity which is believed to affect cancer risk that is related to environmental carcinogen exposure. Diallyl disulfide (DADS) is a naturally occurring organosulfur compound, from garlic (Allium sativum), which exerts anti-neoplasm activity. In this study, we investigated the inhibitory effects of DADS on NAT activity and gene expresseion (NAT mRNA) in human esophagus epidermoid carcinoma CE 81T/ VGH cells. NAT activity was measured by the amounts of N-acetylation of 2-aminofluorene (AF) and non-acetylation of AF by high performance liquid chromatography on cells treated with or without DADS. The amounts of NAT enzymes were examined and analyzed by Western blot. NAT gene expression (NAT mRNA) was examined by polymerase chain reaction and cDNA microarray. DADS decreased the amount of N-acetylation of AF in human esophagus epidermoid carcinoma CE 81T/VGH cells in a dose-dependent manner. Western blot analysis indicated that DADS decreased the levels of NAT protein in CE 81T/VGH cells. PCR and cDNA microarray experiments showed that DADS affected NAT1 mRNA expression in CE 81T/VGH cells. DADS affect NAT activity due to the inhibition of gene expression (NAT 1 mRNA) and the decreasing of the protein levels of NAT in CE 81T/ VGH cells. (c) 2005 Elsevier Ltd. All rights reserved. |
| 關聯: | JOURNAL OF CLINICAL MICROBIOLOGY 43(6):2798-2804 |
| 顯示於類別: | [生物科技學系暨碩士班] 期刊論文
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