Salvianolic acid B enhances in vitro angiogenesis and improves skin flap survival in Sprague-Dawley rats

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Title:	Salvianolic acid B enhances in vitro angiogenesis and improves skin flap survival in Sprague-Dawley rats
Authors:	Lay, IS;Hsieh, CC;Chiu, JH;Shiao, MS;Lui, WY;Wu, CW
Contributors:	北港附設醫院;Natl Yang Ming Univ, Sch Med, Inst Tradit Med, Taipei 112, Taiwan;Vet Gen Hosp, Dept Surg, Div Gen Surg, Taipei 11217, Taiwan;Peikang Hosp, China Med Coll, Yunlin, Taiwan;China Med Coll, Sch Chinese Med, Taichung, Taiwan
Keywords:	Salvia miltiorrhiza;salvianolic acid B;angiogenesis;skin flap;reperfusion injury
Date:	2003
Issue Date:	2010-09-24 14:14:01 (UTC+8)
Publisher:	ACADEMIC PRESS INC ELSEVIER SCIENCE
Abstract:	The main purpose of the National Asthma Education Program was to provide asthma education to school nurses in Taiwan. It was also designed to enhance the knowledge and competence of school nurses in managing the asthmatic problems that children experience while in school. In addition to providing instruction about current asthma management skills, tools, and other relevant information, the program demonstrated the use of the peak flow meter for asthmatic children. A single, 4-hr session conducted in each county and city in Taiwan, the National Asthma Education Program began on August 1, 1999, and ended December 31, 2000. A total of 829 school nurses joined the program, with an overall attendance rate of 74%. Significant effects of this program on nurses' asthma care knowledge and competence and case management efficacy were noted. The participating school nurses' demographics, however, were found to be irrelevant to these effects. Expecting the training activities to help relieve the anxieties of managing asthmatic cases in the school environment, participants reported that the training was of much benefit to them. Development of a teaching program to elevate school nurses' capabilities in asthmatic student care in the school environment and the implications of such a program within Taiwanese schools were also discussed. Insufficient angiogenesis and microcirculatory intravascular clotting have been implicated in the pathophysiology of skin flap failure. Salvianolic acid B (Sal B), isolated from Salvia miltiorrhiza, has been reported to enhance angiogenesis in vitro. This study was aimed to determine the efficacy of Sal B on ischemia-reperfusion injury of the skin flap in Sprague-Dawley rats. Sal B was administered intraperitoneally 2 h before operation, and on the 2nd and 4th days after surgical elevation of an extended epigastric adipocutaneous flap (5 × 7 cm) in ketamine-anesthetized rats. Flap ischemia was achieved by ligating the right superficial epigastric artery and vein and clamping the left superficial epigastric artery and vein for 3 h and then released. Percentage of flap necrosis area (FNA) and plasma levels of aspartate aminotransferase, alanine aminotransferase, creatinine, and malondialdehyde were measured at 7 days after the operation. Animals were divided into six groups, including: vehicle, Sal B low dose (5 mg/kg), Sal B high dose (50 mg/kg) and each with [mesh(+)] or without mesh [mesh(–)] placement. In the three groups with mesh(+), FNA in control flaps was 53.7 ± 6.9%, whereas low-dose and high-dose Sal B significantly improved flap survival with FNA 27.4 ± 3.8% and 25.3 ± 4.3%, respectively (P < 0.05, one-way ANOVA). In the three groups with mesh(–), control flaps were 35.9 ± 4.5%, whereas high-dose Sal B also significantly improved flap survival with FNA 17.9 ± 4.7% (P < 0.05, one-way ANOVA). There were no differences in aspartate aminotransferase, alanine aminotransferase, creatinine, or malondialdehyde between groups. We conclude that Sal B attenuates ischemia-reperfusion injury of skin flap, and provides therapeutic potential in reconstructive plastic surgery.
Relation:	JOURNAL OF SURGICAL RESEARCH 115(2):279-285
Appears in Collections:	[China Medical University Beikang Hospital] Journal articles

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