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請使用永久網址來引用或連結此文件:
http://ir.cmu.edu.tw/ir/handle/310903500/28889
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| 題名: | Relative importance of atherosclerotic risk factors for coronary heart disease in Taiwan |
| 作者: | Chien, KL;Sung, FC;Hsu, HC;Su, TC;Chang, WD;Lee, YT |
| 貢獻者: | 公共衛生學院環醫所;Natl Taiwan Univ Hosp, Dept Internal Med, Taipei 100, Taiwan;Natl Taiwan Univ Hosp, Dept Emergency Med, Taipei 100, Taiwan;Natl Taiwan Univ, Coll Publ Hlth, Inst Prevent Med, Taipei 10764, Taiwan;China Med Univ, Inst Environm Hlth, Taipei, Taiwan |
| 日期: | 2005 |
| 上傳時間: | 2010-09-24 13:59:31 (UTC+8) |
| 出版者: | LIPPINCOTT WILLIAMS & WILKINS |
| 摘要: | A minilibrary was constructed from DOP-PCR products using microdissected Y-chromosomes of Indian muntjac as DNA templates. Two microclones designated as IM-Y4-52 and IM-Y5-7 were obtained from negative screening of all three cervid satellite DNAs (satellites I, II, and IV). These two microclones were 295 and 382 bp in size, respectively, and shared ∼ 70% sequence homology. Southern blot analysis showed that the IM-Y4-52 clone was repetitive in nature with an ∼ 0.32-kb register in HaeIII digest. Sequence comparison revealed no similarities to DNA sequences deposited in the GenBank database, suggesting that the microclone sequences were from a novel satellite DNA family designated as cervid satellite V. A subclone of an Indian muntjac BAC clone which screened positive for IM-Y4-52 had a 3,325-bp insert containing six intact monomers, four deleted monomers, and two partial monomers. The consensus sequence of the monomer was 328 bp in length and shared more than 80% sequence homology with every intact monomer. A zoo blot study using IM-Y4-52 as a probe showed that the strong hybridization with EcoRI digested male genomic DNA of Indian muntjac, Formosan muntjac, Chinese muntjac, sambar deer, and Chinese water deer. Female genomic DNA of Indian muntjac, Chinese water deer, and Formosan muntjac also showed positive hybridization patterns. Satellite V was found to specifically localize to the Y heterochromatin region of the muntjacs, sambar deer, and Chinese water deer and to chromosome 3 of Indian muntjac and the X-chromosome of Chinese water deer. |
| 關聯: | EUROPEAN JOURNAL OF CARDIOVASCULAR PREVENTION & REHABILITATION 12(2):95-101 |
| 顯示於類別: | [環境醫學研究所(已停用)] 期刊論文
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