Abnormal liver function associated with occupational exposure to dimethylformamide and glutathione S-transferase polymorphisms

中國醫藥大學機構典藏 China Medical University Repository, Taiwan > 公共衛生學院 > 環境醫學研究所(已停用) > 期刊論文 > Item 310903500/28886

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題名:	Abnormal liver function associated with occupational exposure to dimethylformamide and glutathione S-transferase polymorphisms
作者:	Luo, JC;Cheng, TJ;Kuo, HW;Chang, MJW
貢獻者:	公共衛生學院環醫所;Chang Gung Med Coll, Dept Publ Hlth, Taoyuan 333, Taiwan;Chang Gung Med Ctr, Dept Occupat Med, Taoyuan, Taiwan;Natl Taiwan Univ, Grad Inst Occupat Med & Ind Hyg, Taipei 10764, Taiwan;China Med Coll, Grad Inst Environm Hlth, Taichung, Taiwan
日期:	2005
上傳時間:	2010-09-24 13:59:25 (UTC+8)
出版者:	TAYLOR & FRANCIS LTD
摘要:	Arylamine carcinogens and drugs are N-acetylated by cytosolic N-acetyltransferase (NAT), which uses acetylcoenzyme A as a cofactor. NAT plays an initial role in the metabolism of these arylamine compounds. 2-Amino-fluorene is one of the arylamine carcinogens which have been demonstrated to undergo N-acetylation in laboratory animals and humans. Our previous study showed that human cancer cell lines (colon cancer, colo 205; liver cancer, Hep G2; bladder cancer, T24; leukemia, HL-60; prostate cancer, LNCaP; osteogenic sarcoma, U-2 OS; malignant melanoma, A375.S2) displayed NAT activity, which was affected by aloe-emodin in human leukemia cells. The purpose of this study was to determine whether aloe-emodin could affect the enzyme activity and gene expression of NAT at the mRNA and protein levels in malignant human melanoma A375.S2 cells. The results showed that aloe-emodin inhibited NAT1 activity (decreased N-acetylation of 2-aminofluorene) in intact cells in a dose-dependent manner. The effect of aloe-emodin on NAT1 at the protein level was determined by Western blotting and the mRNA levels were examined by polymerase chain reaction (PCR) and cDNA microarray. These results clearly indicate that aloe-emodin inhibits the mRNA expression and enzyme activity of NAT1 in A375.S2 cells.
關聯:	BIOMARKERS 10(6):464-474
顯示於類別:	[環境醫學研究所(已停用)] 期刊論文

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