Abnormal liver function associated with occupational exposure to dimethylformamide and glutathione S-transferase polymorphisms

CMUR > College of Public Health > Graduate Institute of Environmental Medicine > Journal articles > Item 310903500/28886

Please use this identifier to cite or link to this item: http://ir.cmu.edu.tw/ir/handle/310903500/28886

Title:	Abnormal liver function associated with occupational exposure to dimethylformamide and glutathione S-transferase polymorphisms
Authors:	Luo, JC;Cheng, TJ;Kuo, HW;Chang, MJW
Contributors:	公共衛生學院環醫所;Chang Gung Med Coll, Dept Publ Hlth, Taoyuan 333, Taiwan;Chang Gung Med Ctr, Dept Occupat Med, Taoyuan, Taiwan;Natl Taiwan Univ, Grad Inst Occupat Med & Ind Hyg, Taipei 10764, Taiwan;China Med Coll, Grad Inst Environm Hlth, Taichung, Taiwan
Date:	2005
Issue Date:	2010-09-24 13:59:25 (UTC+8)
Publisher:	TAYLOR & FRANCIS LTD
Abstract:	Arylamine carcinogens and drugs are N-acetylated by cytosolic N-acetyltransferase (NAT), which uses acetylcoenzyme A as a cofactor. NAT plays an initial role in the metabolism of these arylamine compounds. 2-Amino-fluorene is one of the arylamine carcinogens which have been demonstrated to undergo N-acetylation in laboratory animals and humans. Our previous study showed that human cancer cell lines (colon cancer, colo 205; liver cancer, Hep G2; bladder cancer, T24; leukemia, HL-60; prostate cancer, LNCaP; osteogenic sarcoma, U-2 OS; malignant melanoma, A375.S2) displayed NAT activity, which was affected by aloe-emodin in human leukemia cells. The purpose of this study was to determine whether aloe-emodin could affect the enzyme activity and gene expression of NAT at the mRNA and protein levels in malignant human melanoma A375.S2 cells. The results showed that aloe-emodin inhibited NAT1 activity (decreased N-acetylation of 2-aminofluorene) in intact cells in a dose-dependent manner. The effect of aloe-emodin on NAT1 at the protein level was determined by Western blotting and the mRNA levels were examined by polymerase chain reaction (PCR) and cDNA microarray. These results clearly indicate that aloe-emodin inhibits the mRNA expression and enzyme activity of NAT1 in A375.S2 cells.
Relation:	BIOMARKERS 10(6):464-474
Appears in Collections:	[Graduate Institute of Environmental Medicine] Journal articles

Files in This Item:

There are no files associated with this item.

Loading...