資料載入中.....
|
請使用永久網址來引用或連結此文件:
http://ir.cmu.edu.tw/ir/handle/310903500/28885
|
| 題名: | Determination of 1-hydroxypyrene in children urine using column-switching liquid chromatography and fluorescence detection |
| 作者: | Kuo, CT;Chen, HW;Chen, JL |
| 貢獻者: | 公共衛生學院環醫所;China Med Univ, Inst Environm Hlth, Taichung 404, Taiwan;China Med Univ, Sch Risk Management, Taichung 404, Taiwan;Yuanpei Univ Sci & Technol, Dept Environm Engn & Hlth, Hsinchu 300, Taiwan;Natl Chiao Tung Univ, Inst Environm Engn, Hsinchu 300, Taiwan |
| 日期: | 2004 |
| 上傳時間: | 2010-09-24 13:59:23 (UTC+8) |
| 出版者: | ELSEVIER SCIENCE BV |
| 摘要: | The objective of this study was to establish analytical approaches to quantify 1,3-beta-glucan (1,3-beta-G) in foods. Six food categories including legumes, cereals, tubers, vegetables, fruits, and mushrooms and 17 total items were tested. An extraction procedure was designed to prepare food cold-water soluble, hot-water soluble, cold-alkaline soluble, and hot-alkaline soluble fractions. A fluorescence microassay based on aniline blue dye, which bound specifically to 1,3-beta-G, was developed to measure its content in the food fractions. Curdlan was used as standard to construct the 1,3-beta-G calibration curve, and a linear correlation within a 14 mug/mL concentration range was obtained. This microassay displayed selectivities among various 1,3-beta-G species. Biologically active ones such as pachyman and yeast glucan possessed much stronger fluorescent signals than others such as laminarin and barley glucan. Possible fluorescent interference from food proteins was estimated. This assay tolerated up to 50% of bovine serum albumin in 10 mug/mL curdlan. Analysis of the four food fractions showed that besides the well-known lentinan-containing shiitake, popular foods such as celery, chin-chian leaves, carrot, and radish contained nearly 20% 1,3-beta-G in their total sugar. Soybean dry weight contained 0.8% 1,3-beta-G, which was twice the amount compared to shiitake. Snow mushroom dry weight had the highest 1,3-beta-G content, at 2.5%, and was rich in both water (0.67%) and alkaline soluble (1.87%) forms. In conclusion, this dye-binding fluorescence microassay in conjunction with the extraction procedure can be applied in the prescreening of potential foods rich in functional 1,3-beta-G. |
| 關聯: | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 805(2):187-193 |
| 顯示於類別: | [環境醫學研究所(已停用)] 期刊論文
|
在CMUR中所有的資料項目都受到原著作權保護.
|
