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| 題名: | Prevalence of Bartonella infection in domestic cats in Denmark |
| 作者: | Chomel, BB;Boulouis, HJ;Petersen, H;Kasten, RW;Yamamoto, K;Chang, CC;Gandoin, C;Bouillin, C;Hew, CM |
| 貢獻者: | 公共衛生學院環醫所;Univ Calif Davis, Sch Vet Med, Dept Populat Hlth & Reprod, Davis, CA 95616 USA;Ecole Natl Vet, ENVA, AFSSA, INRA,UMR 956, F-94704 Maisons Alfort, France;Hinnerup Anim Hosp, Dyrlaegegrp Frijsenborg, DK-8382 Hinnerup, Denmark;China Med Coll, Inst Environm Hlth, Dept Publ Hlth, Taichung, Taiwan |
| 日期: | 2002 |
| 上傳時間: | 2010-09-24 13:58:53 (UTC+8) |
| 出版者: | E D P SCIENCES |
| 摘要: | Background: Epidemiological studies have indicated that non-steroidal anti-inflammatory drugs (NSAIDs) can reduce the risk of esophageal squamous cell carcinoma (ESCC) by taking cyclooxygenase (COX) as the target enzyme. The pathophysiological regulation of COX-2 may play a role in carcinogenesis and in disease progression of esophageal carcinoma. Methods: 59 ESCC samples were used to assess COX-2 expression in the tumor cells and four ESCC cell lines to investigate the effects of phorbol myristate acetate (PMA), platelet activating factor (PAF), n-sodium butyrate (n-BT) and interleukin-6 (IL-6) on the expression of COX-2. Expression of COX-2 was determined by immunohistochemistry (IHC) and reverse transcription-polymerase chain reaction (RT-PCR). Production of PGE(2) was measured by a competitive enzyme immunoassay (CEIA). Results: COX-2 expression was detected in 54.2% (32/59) of the pathological sections by IHC. COX-2 expression in ESCC cells was significantly increased following treatment with PAF and n-BT. Increased production of PGE2 was detected in the culture media, and the secreted PGE2 in the culture media was proportional to the increased COX-2 expression. The addition of IL-6 could also enhance COX-2 expression in ESCC cells. While NSAIDs could inhibit enzymatic activity of COX-2, they did not inhibit COX-2 gene expression in ESCC cells. PKC inhibitor, however, could abrogate PMA-induced COX-2 gene expression, but it did not block IL-6-induced COX-2 expression. Conclusions: Our data suggest that COX-2 expression in ESCC cells could be upregulated by PMA, PAF, n-BT and IL-6. Nonetheless, IL-6-induced COX-2 expression could be independent of PKC activation. |
| 關聯: | VETERINARY RESEARCH 33(2):205-213 |
| 顯示於類別: | [環境醫學研究所(已停用)] 期刊論文
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