Disinfection of hospital waste sludge using hypochlorite and chlorine dioxide

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Title:	Disinfection of hospital waste sludge using hypochlorite and chlorine dioxide
Authors:	Tsai, CT;Lin, ST
Contributors:	公共衛生學院環醫所;Providence Univ, Dept Appl Chem, Taichung Hsien 43301, Taiwan;Providence Univ, China Med Coll, Inst Environm Hlth, Taichung Hsien 43301, Taiwan
Date:	1999
Issue Date:	2010-09-24 13:58:07 (UTC+8)
Publisher:	BLACKWELL SCIENCE LTD
Abstract:	Alylamine N-acetyltransferase (NAT) activity in Pseudomonas aeruginosa was inhibited by ellagic acid (EA), a naturally occurring dietary plant phenol. By measuring the acetylation of 2-aminofluorene (2-AF), the NAT activity was determined. In P. aeruginosa ATCC 27853, a NAT activity of 1.37 +/- 0.25 nmol/min/10(10) CFU for intact cell and a NAT activity of 5.92 +/- 0.20 nmol/min/mg protein for cytosolic preparation were measured. EA (ranging from 1 to 0.125 mM) showed a dose-dependent inhibition of NAT activities in the analysis of both intact cell and cytosolic preparations. Enzymatic kinetics were determined and found that EA was a potent non-competitive inhibitor of NAT activity in P. aeruginosa ATCC 27853. EA inhibition of NAT activities in P. aeruginosa ATCC 27853 was time-dependent for at least 4 hrs. These data strongly indicated that EA could suppress NAT activity in P. aeruginosa.
Relation:	JOURNAL OF APPLIED MICROBIOLOGY 86(5):827-833
Appears in Collections:	[Graduate Institute of Environmental Medicine] Journal articles

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