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請使用永久網址來引用或連結此文件:
http://ir.cmu.edu.tw/ir/handle/310903500/28816
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| 題名: | Mood improvement after surgery for obstructive sleep apnea |
| 作者: | Li, HY;Huang, YS;Chen, NH;Fang, TJ;Liu, CY;Wang, PC |
| 貢獻者: | 公共衛生學院公衛系;Cathay Gen Hosp, Dept Otolaryngol, Taipei 106, Taiwan;China Med Univ, Dept Publ Hlth, Taichung, Taiwan;Chang Gung Mem Hosp, Dept Psychiat, Taipei 10591, Taiwan;Chang Gung Mem Hosp, Sleep Ctr, Dept Pulm & Crit Care Med, Taipei 10591, Taiwan;Chang Gung Mem Hosp, Dept Child Psychiat, Taipei 10591, Taiwan;Chang Gung Mem Hosp, Dept Otolaryngol, Taipei, Taiwan |
| 日期: | 2004 |
| 上傳時間: | 2010-09-24 13:55:32 (UTC+8) |
| 出版者: | LIPPINCOTT WILLIAMS & WILKINS |
| 摘要: | Background: Sublingual-swallow immunotherapy in house dust mite-related asthma has a good safety profile and improves respiratory function and bronchial hyperreactivity. Zucchini yellow mosaic virus (ZYMV) is envisaged as a promising viral vector for expressing large quantity of foreign proteins in cucurbit species. Objective: We sought to investigate whether oral feeding of dust mite allergen expressed by ZYMV in a cucurbit species can suppress allergen-induced inflammation and IgE synthesis. Methods: An infectious plant virus clone, p35SZYMV2-26, that contains the full-length cDNA to the genomic RNA of a Taiwan isolate of ZYMV, driven by the cauliflower mosaic virus 35S promoter, was engineered as an in vivo viral vector to express Dermatophagoides pteronyssinus group 5 allergen (Der p 5) in cucurbit species. Female BALB/c mice were intraperitoneally sensitized with Escherichia coli bacteria-expressed Der p 5 and orally treated with the virus-expressed Der p 5 (vDer p 5) extracted from the recombinant virus-infected squash plants. Der p 5-specific immunoglobulins were measured by ELISA, and bronchoalveolar lavage assays were used to measure airway inflammation. Results: Infectivity assays and immunoblotting revealed that large quantities of free-form vDer p 5 are produced in the recombinant virus-infected squash plants. The recombinant virus carried and expressed the Der p 5 allergen in squash plants for at least 1 year after numerous passages. In animal tests, squash extract containing vDer p 5 inhibited Der p 5-specific IgE synthesis and airway inflammation. Conclusion: Our results suggest that oral feeding with allergen produced by the plant viral vector provides a novel approach for the therapy of allergic asthma. |
| 關聯: | LARYNGOSCOPE 114(6):1098-1102 |
| 顯示於類別: | [公共衛生學系暨碩博班] 期刊論文
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