17 beta-estradiol downregulates angiotensin-II-induced endothelin-1 gene expression in rat aortic smooth muscle cells

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Title:	17 beta-estradiol downregulates angiotensin-II-induced endothelin-1 gene expression in rat aortic smooth muscle cells
Authors:	Hong, HJ;Liu, JC;Chan, P;Juan, SH;Loh, SH;Lin, JG;Cheng, TH
Contributors:	中醫學院中醫所;Taipei Med Univ, Wan Fang Hosp, Dept Med, Taipei, Taiwan;China Med Univ, Sch Chinese Med, Taichung, Taiwan;Taipei Med Univ, Wan Fang Hosp, Clin Res Ctr, Taipei, Taiwan;Natl Def Med Ctr, Dept Pharmacol, Taipei, Taiwan;China Med Univ, Inst Chinese Med Sci, Taichung, Taiwan
Date:	2004
Issue Date:	2010-09-24 13:45:44 (UTC+8)
Publisher:	KARGER
Abstract:	In this study, we evaluated the potential apoptosis effects of baicalein on human promyelocytic leukemia HL-60 cells in vitro. Apoptosis induction, cell viability, morphology and caspase-3 activity were then performed to determine by flow cytometric assay, DNA gel electrophoresis, anti-ADP-ribose stain and determination of caspase-3 activity. There is a significant difference in cell death of HL-60 cells that was detected between baicalein-treated and untreated groups. Furthermore, there was a further significant increase in apoptosis induction when cells were treated with baicalein compared to without baicalein treated groups. Flow cytometric assays and DNA fragmentation gel electrophoresis also confirmed baicalein induced apoptosis in HL-60 cells. Baicalein also promoted caspase-3 activity then leading to cleavage of poly-ADP-ribose polymerase finally leading to DNA fragmentation occurrence. Furthermore, the baicalein-induced apoptosis was markedly blocked by the broad-spectrum caspase inhibitor, z-VAD-fmk. Taken together, these results suggest that treatment of human leukemia HL-60 cells with baicalein induced apoptosis through activation of caspase-3 activity. (C) 2003 Elsevier Ltd. All rights reserved.
Relation:	JOURNAL OF BIOMEDICAL SCIENCE 11(1):27-36
Appears in Collections:	[Graduate Institute of Chinese Medical Science] Journal articles

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