Saikosaponin-A induces apoptotic mechanism in human breast MDA-MB-231 and MCF-7 cancer cells

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Title:	Saikosaponin-A induces apoptotic mechanism in human breast MDA-MB-231 and MCF-7 cancer cells
Authors:	Chen, JC;Chang, NW;Chung, JG;Chen, KC
Contributors:	中醫學院中醫所;Show Chwan Mem Hosp, Dept Chinese Med, Changhua, Taiwan;China Med Coll, Res Inst Chinese Med, Taichung, Taiwan;China Med Coll, Sch Chinese Med, Taichung, Taiwan;China Med Coll, Dept Biochem, Taichung, Taiwan;China Med Coll, Dept Microbiol, Taichung, Taiwan
Date:	2003
Issue Date:	2010-09-24 13:44:57 (UTC+8)
Publisher:	WORLD SCIENTIFIC PUBL CO PTE LTD
Abstract:	This study investigated the effects of various concentrations and incubation time intervals of CDA-II (cell differentiation agent: a preparation of human urine) on cell viability, lipid peroxidation and glutathione and its related enzyme activities in rat hepatocytes. Based on the results of lactate dehydrogenase leakage and microscopic examination, treatment with CDA-II significantly elevated the viability of hepatocytes. The level of lipid peroxidation of cells treated with 2.5 or 5 mg/ml CDA-II was lower than the control after 4, 8 and 24 hours of incubation. Intracellular glutathione (GSH) content of cells treated with 0.25-5 mg/ml CDA-II was significantly higher than controls after 8 and 24 hours of incubation. These phenomena are beneficial to the antioxidant capabilities of hepatocytes. Furthermore, treatment of hepatocytes with CDA-II has no effect on the activities of GSH peroxidase, GSH reductase and GSH S-transferase. In conclusion, adequate concentration of CDA-II could enhance the viability and antioxidative capability of hepatocytes.
Relation:	AMERICAN JOURNAL OF CHINESE MEDICINE 31(3):363-377
Appears in Collections:	[Graduate Institute of Chinese Medical Science] Journal articles

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