Evaluation of four prescriptions of Traditional Chinese Medicine: Syh-Mo-Yiin, Guizhi-Fuling-Wan, Shieh-Qing-Wan and Syh-Nih-Sann on experimental acute liver damage in rats

中國醫藥大學機構典藏 China Medical University Repository, Taiwan > 中醫學院 > 中國醫學研究所 > 期刊論文 > Item 310903500/28718

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.cmu.edu.tw/ir/handle/310903500/28718

题名:	Evaluation of four prescriptions of Traditional Chinese Medicine: Syh-Mo-Yiin, Guizhi-Fuling-Wan, Shieh-Qing-Wan and Syh-Nih-Sann on experimental acute liver damage in rats
作者:	Tsai, CC;Kao, CT;Hsu, CT;Lin, CC;Lin, JG
贡献者:	中醫學院中醫所;CHINA MED COLL,RES INST CHINESE MED,TAICHUNG,TAIWAN;CHINA MED COLL,DEPT PATHOL,TAICHUNG,TAIWAN;KAOHSIUNG MED COLL,GRAD INST NAT PROD,KAOHSIUNG,TAIWAN
日期:	1997
上传时间:	2010-09-24 13:43:55 (UTC+8)
出版者:	ELSEVIER SCI IRELAND LTD
摘要:	1 The possible mechanisms of action of the inhibitory effect of abruquinone A on the respiratory burst in rat neutrophils in vitro was investigated. 2 Abruquinone A caused an irreversible and a concentration-dependent inhibition of formylmethionyl-leucyl-phenylalanine (fMLP) plus dihydrocytochalasin B (CB)- and phorbol 12-myristate 13-acetate (PMA)-induced superoxide anion (O-2(.-)) generation with IC50 values of 0.33+/-0.05 mu g ml(-1) and 0.49+/-0.04 mu g ml(-1), respectively. 3 Abruquinone A also inhibited O-2 consumption in neutrophils in response to fMLP/CB and PMA. However, abruquinone A did not scavenge the generated O-2(.-) in xanthine-xanthine oxidase system and during dihydroxyfumaric acid (DHF) autoxidation. 4 Abruquinone A inhibited both the transient elevation of [Ca2+](i) in the absence of [Ca2+](o) (IC50 7.8+/-0.2 mu g ml(-1)) and the generation of inositol trisphosphate (IP3) (IC50 10.6+/-2.0 mu g ml(-1)) in response to fMLP. 5 Abruquinone A did not affect the enzyme activities of neutrophil cytosolic protein kinase C (PKC) and porcine heart protein kinase A (PKA). 6 Abruquinone A had no effect on intracellular guanosine 3':5'-cyclic monophosphate (cyclic GMP) levels but decreased the adenosine 3':5'-cyclic monophosphate (cyclic AMP) levels. 7 The cellular formation of phosphatidic acid (PA) and phosphatidylethanol (PEt) induced by fMLP/CB was inhibited by abruquinone A with IC50 values of 2.2+/-0.6 mu g ml(-1) and 2.5+/-0.3 mu g ml(-1), respectively. Abruquinone A did not inhibit the fMLP/CB-induced protein tyrosine phosphorylation but induced additional phosphotyrosine accumulation on proteins of 73-78 kDa in activated neutrophils. 8 Abruquinone A inhibited both the O-2(.-) generation in PMA-activated neutrophil particulate NADPH oxidase (IC50 0.6+/-0.1 mu g ml(-1)) and the iodonitrotetrazolium violet (INT) reduction in arachidonic acid (AA)-activated cell-free system (IC50 1.5+/-0.2 mu g ml(-1)). 9 Collectively, these results indicate that the inhibition of respiratory burst in rat neutrophils by abruquinone A is mediated partly by the blockade of phospholipase C (PLC) and phospholipase D (PLD) pathways, and by suppressing the function of NADPH oxidase through the interruption of electron transport.
關聯:	JOURNAL OF ETHNOPHARMACOLOGY 55(3):213-222
显示于类别:	[中國醫學研究所] 期刊論文

文件中的档案:

没有与此文件相关的档案.

在CMUR中所有的数据项都受到原著作权保护.

TAIR相关文章

数据加载中.....