Anticonvulsant effect of Uncaria rhynchophylla (Miq) Jack. in rats with kainic acid-induced epileptic seizure

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Title:	Anticonvulsant effect of Uncaria rhynchophylla (Miq) Jack. in rats with kainic acid-induced epileptic seizure
Authors:	Hsieh, CL;Chen, MF;Li, TC;Li, SC;Tang, NY;Hsieh, CT;Pon, CZ;Lin, JG
Contributors:	中醫學院中醫系;China Med Coll, Sch Chinese Med, Taichung, Taiwan
Date:	1999
Issue Date:	2010-09-24 13:41:24 (UTC+8)
Publisher:	INST ADV RES ASIAN SCI & MED
Abstract:	Berberine was used to determine loss of viable cells and inhibition of arylamine Nacetyltransferase (NAT) activity in a human colon tumor (adenocarcinoma) cell line. The viable cells were determined by trypan blue exclusion under a light microscope. The NAT activity was measured by high performance liquid chromatography for the amounts of N-acetyl-2-aminofluorene (AAF), N-acetyl-p-aminobenzoic acid (N-Ac-PABA), and the remaining 2-aminofluorene (AF) and p-aminobenzoic acid (PABA). The viability and NAT activity in a human colon tumor cell line was inhibited by berberine in a dose-dependent manner, i.e., the higher the concentration of berberine, the higher the inhibition of NAT activity and cell death. The NAT activities measured in the intact human colon tumor cells were decreased over 50% by AAF and NAc-PABA production from acetylation of AF and PABA. The apparent values of Km and Vmax of NAT from colon tumor cells were also inhibited by berberine in cytosols and in intact cells. This report is the first to show that berberine did affect human colon tumor cell NAT activity.
Relation:	AMERICAN JOURNAL OF CHINESE MEDICINE 27(2):257-264
Appears in Collections:	[School of Chinese Medicine] Journal articles

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