Increase of anti-oxidation by exercise in the liver of obese Zucker rats

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Title:	Increase of anti-oxidation by exercise in the liver of obese Zucker rats
Authors:	Chang, SP;Chen, YH;Chang, WC;Liu, IM;Cheng, JT
Contributors:	中國醫藥大學;Natl Cheng Kung Univ, Coll Med, Dept Pharmacol, Tainan 70101, Taiwan;China Med Univ, Chung Tai Inst Hlth Sci & Technol, Taichung, Taiwan;China Med Univ, Dept Sports Med, Taichung, Taiwan;Natl Cheng Kung Univ, Tajen Inst Technol, Tainan, Taiwan
Date:	2004
Issue Date:	2010-09-24 13:39:41 (UTC+8)
Publisher:	BLACKWELL PUBLISHING ASIA
Abstract:	Genistein, an isoflavonoid natural product, is widely used to inhibit protein tyrosine kinase (PTK). In the present study, we investigated the possible influence of genistein on alpha(1)-adrenoceptors (AR) in cultured C2C12 cells. Genistein enhanced the uptake of radioactive glucose into C2C12 cells in a concentration-dependent manner. Similar results were also observed in samples treated with daidzein, the inactive congener for PTK inhibition. The effect of genistein on alpha(1)-AR was further characterized using the displacement of [H-3]prazosin binding in C2C12 cells. The increase in radioactive glucose uptake by genistein was abolished by RS17053 at a concentration sufficient to block alpha(1A)-AR. The pharmacological inhibition of phospholipase C (PLC) by U73122 resulted in a concentration-dependent reduction of genistein-stimulated glucose uptake in C2C12 cells. This inhibition by U73122 was specific because the inactive congener, U73343, failed to modify the action of genistein. Moreover, genistein can activate alpha(1A)-AR at a concentration (I mumol/L) lower than that (50 mumol/L) needed to abolish the insulin-stimulated phosphorylation of PTK. The obtained data indicate an activation Of alpha(1A)-AR by genistein to increase the glucose uptake into C2C12 cells and this supports the application of genistein as a TK inhibitor.
Relation:	CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY 31(8):506-511
Appears in Collections:	[China Medical University] Journal articles

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