 |
English
|
正體中文
|
简体中文
|
全文筆數/總筆數 : 29490/55136 (53%)
造訪人次 : 1902377
線上人數 : 305
|
|
|
資料載入中.....
|
請使用永久網址來引用或連結此文件:
http://ir.cmu.edu.tw/ir/handle/310903500/28666
|
| 題名: | Heroes of SARS: professional roles and ethics of health care workers |
| 作者: | Hsin, DHC;Macer, DRJ |
| 貢獻者: | 中國醫藥大學;Univ Tsukuba, Inst Biol Sci, Doctoral Program Life & Environm Sci, Tsukuba, Ibaraki 3058572, Japan;China Med Univ, Taichung 40421, Taiwan |
| 日期: | 2004 |
| 上傳時間: | 2010-09-24 13:39:39 (UTC+8) |
| 出版者: | W B SAUNDERS CO LTD |
| 摘要: | Caveolin-1, an integral membrane protein of caveolae found in many cell types, has been suggested as a candidate tumor suppressor. However, the mechanism underlying caveolin-1 decreased expression is not fully understood. The purpose of this study is to investigate the role of aberrant promoter methylation in the regulation of caveolin-1 gene in breast cancer correlated with clinical findings. We used methylation specific PCR, direct sequencing and immunohistochemistry stain methods to explore the role of caveolin-1 gene in the development of breast cancer. We demonstrated that 14 of 55 cases (25.5%) and 4 of 55 cases (7.3%) had methylated CpG-island on caveolin-1 promoter in cancerous and non-cancerous cells, respectively. The frequency of aberrant promoter methylation of breast cancer tissues was significant higher than non-cancerous tissues (p<0.05). There were four types of methylation pattern of caveolin-1 gene in the breast cancer tissues. No mutation but one polymorphism GAC-->GAT at codon 82 was found in the whole exonic sequences of caveolin-1 gene. The methylation status of caveolin-1 gene had no clear relationship with age, cell grade, stage of tumor, and status of estrogen receptor, p53 and c-erbB2 in the breast cancer tissues. However, in breast tissue with aberrant promoter methylation of caveolin-1 gene, the presence of progesterone receptor showed borderline statistic difference compared to unmethylated promoter (p=0.11). Immunohistochemistry demonstrated that expression of caveolin-1 gene correlated with aberrant promoter methylation status in sporadic breast cancer tissues. Our findings suggest that aberrant promoter methylation of caveolin-1 gene is associated with inactivation of expression. This process occurs in the precancerous stage and may play an important role in the development of breast cancer. |
| 關聯: | JOURNAL OF INFECTION 49(3):210-215 |
| 顯示於類別: | [中國醫藥大學] 期刊論文
|
文件中的檔案:
| 檔案 |
大小 | 格式 | 瀏覽次數 |
|---|
| 0Kb | Unknown | 578 | 檢視/開啟 |
|
在CMUR中所有的資料項目都受到原著作權保護.
|
